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CC BY-NC-ND V4.0, Open Access
Journal article
Industrial production and functional profiling of probiotic Pediococcus acidilactici 72 N for potential use as a swine feed additive
Scientific reports, Vol.15(1), 14940
2025
Abstract
This study aimed to achieve industrial-scale production of the Thai native swine-derived probiotic strain Pediococcus acidilactici 72 N (P72N) using a cost-effective, food-grade modified medium, and to assess the efficacy of this medium by evaluating the probiotic’s functional characteristics and metabolomic profile. Conventional and statistical optimization techniques were used to screen food-grade carbon sources (glucose, dextrose, sucrose) and nitrogen sources (beef extract, yeast extract, sweet whey) to develop the optimal formulation. The final medium contained 10 g/L dextrose, 45 g/L yeast extract, 5 g/L sodium acetate, 2 g/L ammonium citrate, 2 g/L di-potassium hydrogen phosphate, 1 g/L Tween 80, 0.1 g/L magnesium sulfate, and 0.05 g/L manganese sulfate. This formulation achieved significantly higher viable cell counts compared to commercial MRS medium. Scale-up fermentation in 5 L and 50 L fermenters under controlled conditions (37 °C, pH 6.5, 120 rpm) yielded viable cell counts exceeding 9.60 log CFU/mL within 12 h, reducing production costs by 67–86%. P72N in the modified medium demonstrated improved tolerance to environmental stresses. Metabolomic analysis revealed that P72N produced a variety of bioactive metabolites, particularly 1,4-dihydroxy-2-naphthoic acid (1,4-DHNA) and indolelactic acid (ILA) which were detected in higher levels in the modified medium, demonstrating its suitability for industrial production of P72N as a potential feed additive for swine farming.
Details
- Title
- Industrial production and functional profiling of probiotic Pediococcus acidilactici 72 N for potential use as a swine feed additive
- Authors/Creators
- Nay Zin Myo - Chulalongkorn UniversityRatchnida Kamwa - Chulalongkorn UniversityBenjamas Khurajog - Chulalongkorn UniversityPawiya Pupa - Chulalongkorn UniversityWandee Sirichokchatchawan - Chulalongkorn UniversityDavid J. Hampson - School of Veterinary Medicine, Murdoch UniversityNuvee Prapasarakul - Chulalongkorn University
- Publication Details
- Scientific reports, Vol.15(1), 14940
- Publisher
- Nature Publishing Group UK
- Number of pages
- 17
- Grant note
- the Secondary Century Fund (C2F) grants for Doctoral Scholarship CRP6605031420 / the Agricultural Research Development Agency, Thailand the 90th Anniversary of Chulalongkorn University Scholarship (Ratchadaphiseksomphot Endowment Fund), Chulalongkorn University
- Identifiers
- 991005774133407891
- Copyright
- © The Author(s) 2025
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.120 Inflammatory Bowel Diseases & Infections
- 1.120.267 Lactic Acid Bacteria
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- ESI research areas
- Microbiology