Inflammation induces α1-adrenoceptor expression in peripheral blood mononuclear cells of patients with complex regional pain syndrome

Linda K. Wijaya; Philip A. Stumbles; Philip M. Finch; Peter D. Drummond

doi:10.1016/j.bbi.2023.10.015

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Inflammation induces α1-adrenoceptor expression in peripheral blood mononuclear cells of patients with complex regional pain syndrome

Journal article

Open access

Peer reviewed

Inflammation induces α1-adrenoceptor expression in peripheral blood mononuclear cells of patients with complex regional pain syndrome

Linda K. Wijaya, Philip A. Stumbles, Philip M. Finch and Peter D. Drummond

Brain, behavior, and immunity, Vol.115, pp.201-208

2024

DOI: https://doi.org/10.1016/j.bbi.2023.10.015

PMID: 37848097

Appears in Open Access via Read & Publish Agreements

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Abstract

Complex regional pain syndrome

Immunoglobulin G

Inflammation

Interleukin-6

Peripheral blood mononuclear cells

α1-adrenoceptors

Persistent regional and systemic inflammation may promote pain and hyperalgesia in complex regional pain syndrome (CRPS). In this study, we investigated whether stimulation of α1-adrenoceptors (α1-AR) on peripheral blood mononuclear cells (PBMC) might contribute to this inflammatory state. PBMC were isolated from venous blood collected from 21 CRPS patients and 21 sex and age-matched controls. Lipopolysaccharide (LPS), a bacterial toxin, was administered to cultured PBMC for 24 h to trigger inflammation. Exposure to LPS resulted in heightened gene expression of α1-AR subtype B (α1B-AR) in PBMC of CRPS patients relative to controls. Interleukin (IL)-1β and IL-6 levels did not change when the α1-AR agonist phenylephrine was administered to naïve PBMC. However, α1-AR stimulation following LPS treatment increased IL-6 mRNA and protein levels in PBMC of patients and controls. To investigate the possible consequence of heightened IL-6 levels on immunoglobulin G antibody production, PBMC were stimulated with CD40 ligand and IL-21 to generate plasmablasts (B cells that secrete antibodies). This response was similar in patients and controls. Adding IL-6 to the cell culture medium increased plasmablast differentiation in controls and antibody production both in patients and controls. These findings suggest that the inflammatory cascade associated with elevated levels of IL-6 may generate α1B-AR expression in CRPS PBMC. A reciprocal interaction between heightened α1-AR expression in PBMC and IL-6 secretion may contribute to systemic inflammation and antibody production in CRPS.

Details

Title: Inflammation induces α1-adrenoceptor expression in peripheral blood mononuclear cells of patients with complex regional pain syndrome
Authors/Creators: Linda K. Wijaya - School of Psychology, College of Health and Education, Murdoch University, 90 South St, Perth, Western Australia 6150, Australia
Philip A. Stumbles - Murdoch University
Philip M. Finch - Murdoch University
Peter D. Drummond - Murdoch University, Centre for Healthy Ageing
Publication Details: Brain, behavior, and immunity, Vol.115, pp.201-208
Publisher: Elsevier Inc
Identifiers: 991005609660707891
Murdoch Affiliation: School of Psychology; Centre for Healthy Ageing
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.43 Anesthesiology; 1.43.1905 Complex Regional Pain Syndrome
Web Of Science research areas: Immunology; Neurosciences; Psychiatry
ESI research areas: Neuroscience & Behavior