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Journal article
Influences of Bacillus pumilus SA388 as an environmentally friendly antibiotic alternative on growth performance, blood biochemistry, immunology, cecal microbiota, and meat quality in broiler chickens
Poultry science, Vol.103(11), 104115
2024
Abstract
The widespread use of antibiotics causes the development of antibiotic-resistant bacterial strains, which have a severe impact on poultry productivity and human health. As a result, research is continuing to develop safe natural antibiotic alternatives. In the current study, Bacillus pumilus SA388 was isolated from the chicken feces and confirmed to be a probiotic. The selected strain was tested for its antimutagenic and antioxidant capabilities before being employed as a probiotic food supplement and antibiotic alternative. The effect of B. pumilus SA388 impact on broiler chickens' growth performance, gut microbiome, blood biochemical markers, immunological response, and meat quality was also studied. B. pumilus SA388 showed significant bactericidal activity against Streptococcus pyogenes, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Klebsiella pneumonia. A total of 200 chickens were used in the present study, divided equally among four experimental groups (ten birds per group with 5 replicates): group 1 (control, G1) received a basal diet without B. pumilus SA388, group 2 (G2) received a basal diet supplemented with 0.4 mg/kg of B. pumilus SA388, group 3 (G3) received a basal diet supplemented with 0.8 mg/kg of B. pumilus SA388, and group 4 (G4) received a basal diet supplemented with 1.6 mg/kg of B. pumilus SA388. Over 35 d, the B. pumilus SA388-supplemented groups outperformed the G1 in terms of body weight gain, performance index, and feed conversion ratio, with a preference for the G4 treatment. The levels of alanine aminotransferase (ALT), aspartate transaminase (AST), low-density lipoprotein (LDL), and total cholesterol decreased significantly (P < 0.05) with increasing B. pumilus SA388 dosages compared to the control G1 group. Dietary supplementation of B. pumilus SA388 at 1.6 mg/kg (G4) significantly (P < 0.05) resulted in improved lipid profile, immunological response, thyroid function, and gut microbiota compared to the control group (G1). Compared to the broilers in the control treatment (G1), the addition of B. pumilus SA388 to broilers in G4 significantly (P < 0.05) enhanced juiciness, tenderness, aroma, and taste. Adding B. pumilus SA388 to chicken feed at different doses significantly (P < 0.05) decreased average feed intake while increasing economic and relative efficiency measures. In conclusion, B. pumilus SA388 has been proven to be an effective antibiotic and nutritional supplement.
Details
- Title
- Influences of Bacillus pumilus SA388 as an environmentally friendly antibiotic alternative on growth performance, blood biochemistry, immunology, cecal microbiota, and meat quality in broiler chickens
- Authors/Creators
- Eman A. Beyari - King Abdulaziz UniversityNaheda M. Alshammari - King Abdulaziz UniversitySoha A. Alamoudi - King Abdulaziz UniversityAlaa S. Mohamed - Zagazig UniversityLamaia R. Altarjami - King Abdulaziz UniversityRoua S. Baty - Taif UniversityNada Alqadri - Taif UniversityAshwaq M. Al-Nazawi - Jazan UniversityAhmed M. Saad - Zagazig UniversityTaha F. Taha - Zagazig UniversityMohamed T. El-Saadony - Zagazig UniversityKhaled A. El-Tarabily - United Arab Emirates UniversityNadeen G. Mostafa - Zagazig University
- Publication Details
- Poultry science, Vol.103(11), 104115
- Publisher
- Elsevier Inc. on behalf of Poultry Science Association Inc.
- Number of pages
- 15
- Grant note
- Taif University, Saudi Arabia: TU-DSPP-2024-199 Abu Dhabi Department of Education and Knowledge: 21S105 UAEU Program for Advanced Research: 12S169 Taif University, Taif, Saudi Arabia: TU-DSPP-2024-199
The authors extend their appreciation to Taif University, Saudi Arabia, for supporting this work through project number (TU-DSPP-2024-199). This research was funded by Abu Dhabi Department of Education and Knowledge, grant number 21S105 to KE-T; and UAEU Program for Advanced Research grant number 12S169 to KE-T.Funding: This research was funded by Taif University, Taif, Saudi Arabia (TU-DSPP-2024-199).Author Contributions: Conceptualization, E.A.B., N. M.A., S.A.A., A.S.M., L.R.A., R.S.B., N.A., and K.A. E.-T., formal analysis, A.M.A.-N., A.M.S., T.F.T., M.T. E.-S., and N.G.M., investigation, E.A.B., N.M.A., S.A. A., A.S.M., L.R.A., R.S.B., and N.A., data curation, A. M.A.-N., A.M.S., T.F.T., M.T.E.-S., and N.G.M., writ-ing original draft preparation, E.A.B., N.M.A., S.A.A., A.S.M., L.R.A., R.S.B., N.A., and K.A.E.-T., writing final manuscript and editing, A.M.A.-N., A.M.S., T.F. T., M.T.E.-S., N.G.M., and K.A.E.-T., visualization and methodology, E.A.B., N.M.A., S.A.A., A.S.M., L.R.A., R.S.B., N.A., A.M.A.-N., A.M.S., T.F.T., M.T.E.-S., and N.G.M. All authors have read and agreed to the published version of the manuscript.
- Identifiers
- 991005687702907891
- Copyright
- © 2024 The Authors.
- Murdoch Affiliation
- Centre for Terrestrial Ecosystem Science and Sustainability
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.51 Dairy & Animal Sciences
- 3.51.208 Poultry Nutrition
- Web Of Science research areas
- Agriculture, Dairy & Animal Science
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- Plant & Animal Science