Journal article
Insights into the micelle-induced β-hairpin-to-α-helix transition of a LytA-derived peptide by photo-CIDNP spectroscopy
International Journal of Molecular Sciences, Vol.22(13), Article 6666
2021
Abstract
A choline-binding module from pneumococcal LytA autolysin, LytA239–252, was reported to have a highly stable nativelike β-hairpin in aqueous solution, which turns into a stable amphipathic α-helix in the presence of micelles. Here, we aim to obtain insights into this DPC-micelle triggered β-hairpin-to-α-helix conformational transition using photo-CIDNP NMR experiments. Our results illustrate the dependency between photo-CIDNP phenomena and the light intensity in the sample volume, showing that the use of smaller-diameter (2.5 mm) NMR tubes instead of the conventional 5 mm ones enables more efficient illumination for our laser-diode light setup. Photo-CIDNP experiments reveal different solvent accessibility for the two tyrosine residues, Y249 and Y250, the latter being less accessible to the solvent. The cross-polarization effects of these two tyrosine residues of LytA239–252 allow for deeper insights and evidence their different behavior, showing that the Y250 aromatic side chain is involved in a stronger interaction with DPC micelles than Y249 is. These results can be interpreted in terms of the DPC micelle disrupting the aromatic stacking between W241 and Y250 present in the nativelike β-hairpin, hence initiating conversion towards the α-helix structure. Our photo-CIDNP methodology represents a powerful tool for observing residue-level information in switch peptides that is difficult to obtain by other spectroscopic techniques.
Details
- Title
- Insights into the micelle-induced β-hairpin-to-α-helix transition of a LytA-derived peptide by photo-CIDNP spectroscopy
- Authors/Creators
- M.V. Gomez (Author/Creator) - University of Castilla-La ManchaM. Ruiz-Castañeda (Author/Creator) - University of Castilla-La ManchaP. Nitschke (Author/Creator) - University of RegensburgR.M. Gschwind (Author/Creator) - University of RegensburgM.A. Jiménez (Author/Creator) - Instituto de Química Física Blas Cabrera
- Publication Details
- International Journal of Molecular Sciences, Vol.22(13), Article 6666
- Publisher
- MDPI
- Identifiers
- 991005543404507891
- Copyright
- © 2021 by the authors
- Murdoch Affiliation
- Australian National Phenome Centre; Health Futures Institute
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.234 Photochemistry
- 2.234.1149 Photoresponsive Polymers
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry