Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.

S. Miyake; M. Soh; M.N. Azman; S.Y. Ngoh; L. Orbán; H. Seedorf

doi:10.1007/s10482-020-01391-9

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Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.

Journal article

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Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.

S. Miyake, M. Soh, M.N. Azman, S.Y. Ngoh, L. Orbán and H. Seedorf

Antonie van Leeuwenhoek

2020

DOI: https://doi.org/10.1007/s10482-020-01391-9

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Abstract

Outbreaks of diseases in farmed fish remain a recurring problem despite the development of vaccines and improved hygiene standards on aquaculture farms. One commonly observed bacterial disease in tropical aquaculture of the South-East Asian region is tenacibaculosis, which is attributed to members of the genus Tenacibaculum (family Flavobacteriaceae, phylum Bacteroidetes), most notably Tenacibaculum maritimum. The impact of tenacibaculosis on the fish microbiota remains poorly understood. In this study, we analysed the microbiota of different tissues of commercially reared Asian seabass (Lates calcarifer) that showed symptoms of tenacibaculosis and compared the microbial communities to those of healthy and experimentally infected fish that were exposed to diseased farmed fish. The relative abundance of Tenacibaculum species in experimentally infected fish was significantly lower than in commercially reared diseased fish and revealed a higher prevalence of different Tenacibaculum species. One isolated strain, TLL-A2T, shares 98.7% 16S rRNA gene identity with Tenacibaculum mesophilum DSM 13764T. The genome of strain TLL-A2T was sequenced and compared to that of T. mesophilum DSM 13764T. Analysis of average nucleotide identity and comparative genome analysis revealed only 92% identity between T. mesophilum DSM 13764T and strain TLL-A2T and differences between the two strains in predicted carbohydrate activating enzymes respectively. Phenotypic comparison between strain TLL-A2T and T. mesophilum DSM 13764T indicated additional differences, such as growth response at different salt concentrations. Based on molecular and phenotypic differences, strain TLL-A2T (=DSM 106434T, KCTC 62393T) is proposed as the type strain of Tenacibaculum singaporense sp. nov.

Details

Title: Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.
Authors/Creators: S. Miyake (Author/Creator) - Temasek Life Sciences Laboratory
M. Soh (Author/Creator) - Temasek Life Sciences Laboratory
M.N. Azman (Author/Creator) - Temasek Life Sciences Laboratory
S.Y. Ngoh (Author/Creator) - Agri-Food and Veterinary Authority of Singapore
L. Orbán (Author/Creator) - Murdoch University
H. Seedorf (Author/Creator) - National University of Singapore
Publication Details: Antonie van Leeuwenhoek
Publisher: Kluwer Academic Publishers
Identifiers: 991005540909407891
Copyright: © 2020 Springer Nature Switzerland AG
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.83 Bioengineering; 3.83.167 Microbial Diversity
Web Of Science research areas: Microbiology
ESI research areas: Microbiology