Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription

M.H. Cake; K.K.W. Ho; L. Shelly; E. Milward; G.C.T. Yeoh

doi:10.1111/j.1432-1033.1989.tb14849.x

Back

Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription

Journal article

Peer reviewed

Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription

M.H. Cake, K.K.W. Ho, L. Shelly, E. Milward and G.C.T. Yeoh

European Journal of Biochemistry, Vol.182(2), pp.429-435

1989

DOI: https://doi.org/10.1111/j.1432-1033.1989.tb14849.x

Files and links (1)

url

Link to Published Version *Subscription may be requiredView

Abstract

Previous studies have shown that insulin depresses the induction of tyrosine aminotransferase by glucocorticoids in cultured fetal rat hepatocytes. However, the site at which this inhibitory effect is exerted was not elucidated, since only enzyme activity was determined in such studies. Therefore, the effet of insulin on tyrosine aminotransferase synthesis, the level of its mRNA as well as the rate of transcription of the gene in isolated nuclei have been determined. The results obtained indicate that in cultures exposed to dexamethasone, Bt2cAMP, insulin and combinations of these additives, there is an excellent correlation between the enzyme activity, enzyme synthesis and the level of mRNA. Run-on transcription experiments indicate that the reduction in the level of mRNA by insulin in dexamethasone-supplemented cultures is the result of a diminished rate of gene transcription.

Details

Title: Insulin antagonism of dexamethasone induction of tyrosine aminotransferase in cultured fetal hepatocytes. A correlation between enzyme activity, synthesis, level of messenger RNA and transcription
Authors/Creators: M.H. Cake (Author/Creator)
K.K.W. Ho (Author/Creator)
L. Shelly (Author/Creator)
E. Milward (Author/Creator)
G.C.T. Yeoh (Author/Creator)
Publication Details: European Journal of Biochemistry, Vol.182(2), pp.429-435
Publisher: Blackwell Publishing
Identifiers: 991005540751907891
Murdoch Affiliation: School of Biological and Environmental Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

25 Record Views

21 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.26 Diabetes; 1.26.1876 Adipogenesis
Web Of Science research areas: Biochemistry & Molecular Biology
ESI research areas: Biology & Biochemistry