Journal article
Integrated fecal microbiome–metabolome signatures reflect stress and serotonin metabolism in irritable bowel syndrome
Gut Microbes, Vol.14(1), Art. e2063016
2022
Abstract
To gain insight into the complex microbiome-gut-brain axis in irritable bowel syndrome (IBS), several modalities of biological and clinical data must be combined. We aimed to identify profiles of fecal microbiota and metabolites associated with IBS and to delineate specific phenotypes of IBS that represent potential pathophysiological mechanisms. Fecal metabolites were measured using proton nuclear magnetic resonance (1H-NMR) spectroscopy and gut microbiome using shotgun metagenomic sequencing (MGS) in a combined dataset of 142 IBS patients and 120 healthy controls (HCs) with extensive clinical, biological and phenotype information. Data were analyzed using support vector classification and regression and kernel t-SNE. Microbiome and metabolome profiles could distinguish IBS and HC with an area-under-the-receiver-operator-curve of 77.3% and 79.5%, respectively, but this could be improved by combining microbiota and metabolites to 83.6%. No significant differences in predictive ability of the microbiome–metabolome data were observed between the three classical, stool pattern-based, IBS subtypes. However, unsupervised clustering showed distinct subsets of IBS patients based on fecal microbiome–metabolome data. These clusters could be related plasma levels of serotonin and its metabolite 5-hydroxyindoleacetate, effects of psychological stress on gastrointestinal (GI) symptoms, onset of IBS after stressful events, medical history of previous abdominal surgery, dietary caloric intake and IBS symptom duration. Furthermore, pathways in metabolic reaction networks were integrated with microbiota data, that reflect the host-microbiome interactions in IBS. The identified microbiome–metabolome signatures for IBS, associated with altered serotonin metabolism and unfavorable stress response related to GI symptoms, support the microbiota-gut-brain link in the pathogenesis of IBS.
Details
- Title
- Integrated fecal microbiome–metabolome signatures reflect stress and serotonin metabolism in irritable bowel syndrome
- Authors/Creators
- Z. Mujagic (Author/Creator) - Maastricht UniversityM. Kasapi (Author/Creator) - Imperial College LondonD.M. Jonkers (Author/Creator) - Maastricht UniversityI. Garcia-Perez (Author/Creator) - Imperial College LondonL. Vork (Author/Creator) - Maastricht UniversityZ.Z.R.M. Weerts (Author/Creator) - Maastricht UniversityJ.I. Serrano-Contreras (Author/Creator) - Imperial College LondonA. Zhernakova (Author/Creator) - University Medical Center GroningenA. Kurilshikov (Author/Creator) - University Medical Center GroningenJ. Scotcher (Author/Creator) - Imperial College LondonE. Holmes (Author/Creator) - Imperial College LondonC. Wijmenga (Author/Creator) - University Medical Center GroningenD. Keszthelyi (Author/Creator) - Maastricht UniversityJ.K. Nicholson (Author/Creator) - The Australian National Phenome Center, Harry Perkins Institute, Murdoch University, Perth, Australia.J.M Posma (Author/Creator) - Imperial College LondonA.A. Masclee (Author/Creator) - Maastricht University
- Publication Details
- Gut Microbes, Vol.14(1), Art. e2063016
- Publisher
- Taylor & Francis
- Identifiers
- 991005540594607891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.95 Gastrointestinal & Esophageal Diseases
- 1.95.723 IBS & Functional Disorders
- Web Of Science research areas
- Gastroenterology & Hepatology
- Microbiology
- ESI research areas
- Microbiology