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Journal article
Integrated multi‐omics analysis reveals drought stress response mechanism in chickpea ( Cicer arietinum L.)
The plant genome, Vol.17(1), e20337
2024
PMID: 37165696
Abstract
Drought is one of the major constraints limiting chickpea productivity. To unravel complex mechanisms regulating drought response in chickpea, we generated transcriptomics, proteomics, and metabolomics datasets from root tissues of four contrasting drought-responsive chickpea genotypes: ICC 4958, JG 11, and JG 11+ (drought-tolerant), and ICC 1882 (drought-sensitive) under control and drought stress conditions. Integration of transcriptomics and proteomics data identified enriched hub proteins encoding isoflavone 4′-O-methyltransferase, UDP-d-glucose/UDP-d-galactose 4-epimerase, and delta-1-pyrroline-5-carboxylate synthetase. These proteins highlighted the involvement of pathways such as antibiotic biosynthesis, galactose metabolism, and isoflavonoid biosynthesis in activating drought stress response mechanisms. Subsequently, the integration of metabolomics data identified six metabolites (fructose, galactose, glucose, myoinositol, galactinol, and raffinose) that showed a significant correlation with galactose metabolism. Integration of root-omics data also revealed some key candidate genes underlying the drought-responsive “QTL-hotspot” region. These results provided key insights into complex molecular mechanisms underlying drought stress response in chickpea.
Core Ideas
• Multi-omics analysis of chickpea roots revealed complex molecular mechanisms underpinning drought stress response.
• Integration of transcriptome and proteome data uncovered hub proteins involved in drought stress response pathways.
• Metabolomic profiling identified six metabolites showing a significant correlation with galactose metabolism.
• Transcriptome-proteome integration revealed prominent differential expression of key genes underlying the “QTL-hotspot” region.
Details
- Title
- Integrated multi‐omics analysis reveals drought stress response mechanism in chickpea ( Cicer arietinum L.)
- Authors/Creators
- Himabindu Kudapa - International Crops Research Institute for the Semi-Arid TropicsArindam Ghatak - University of ViennaRutwik Barmukh - Murdoch University, Centre for Crop and Food InnovationPalak Chaturvedi - University of ViennaAamir Khan - International Crops Research Institute for the Semi-Arid TropicsSandip Kale - Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)Lena Fragner - University of ViennaAnnapurna Chitikineni - Murdoch UniversityWolfram Weckwerth - University of ViennaRajeev K. Varshney - Murdoch University, Food Futures Institute
- Publication Details
- The plant genome, Vol.17(1), e20337
- Publisher
- Wiley Periodicals LLC on behalf of Crop Science Society of America.
- Identifiers
- 991005649567707891
- Copyright
- © 2023 The Authors.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Genetics & Heredity
- Plant Sciences
- ESI research areas
- Plant & Animal Science