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Journal article
Integrating Serological and Genomic Data to Elucidate Lumpy Skin Disease Virus Diversity in Cattle from Bangladesh
Viruses, Vol.17(8), 1126
2025
Abstract
Lumpy skin disease virus (LSDV), a transboundary pathogen threatening cattle health in South and Southeast Asia, presents growing challenges for disease control. This study combined serological, molecular, and genomic approaches to investigate LSDV in Barura Upazila, Bangladesh. Serological screening of 424 cattle using a commercial ELISA revealed a high seroprevalence of 55.5% (95% CI: 50.7–60.3), indicating widespread exposure. Although differences were observed by age and breed, no significant associations were found with seropositivity, suggesting broad regional circulation. Real-time PCR confirmed LSDV DNA in all 20 clinically infected animals, with consistent P32 gene amplification. Two samples with low Cq values underwent whole-genome sequencing. The complete genomes of LSDV-L2/2024 and LSDV-L3/2024 showed >99.6% identity with the reference strain LSDV-29, yet carried unique genomic features, including truncated or variant ORFs and immune-related gene differences. Phylogenetic analysis of the DNA polymerase gene revealed distinct clustering: L2/2024 aligned with South Asian isolates, while L3/2024 grouped with strains from Africa, the Middle East, and Europe. These results highlight co-circulation of genetically diverse strains and possible cross-regional introductions. Overall, our findings underscore the evolutionary plasticity of LSDV and the critical need for ongoing genomic surveillance to guide targeted vaccine development and disease control strategies.
Details
- Title
- Integrating Serological and Genomic Data to Elucidate Lumpy Skin Disease Virus Diversity in Cattle from Bangladesh
- Authors/Creators
- Nasrin Sultana Tonu - Bangladesh Livestock Research InstituteSajedul Hayat - Bangladesh Livestock Research InstituteShukes Chandra Badhy - Bangladesh Livestock Research InstituteSalima Ferdows - Bangladesh Livestock Research InstituteMd. Golam Azam Chowdhury - Bangladesh Livestock Research InstituteBabu Kanti Nath - Charles Sturt UniversityMd Safiul Alam Bhuiyan - Universiti of Malaysia SabahMuhammad Jasim Uddin - Murdoch University, Centre for Animal Production and HealthSuman Das GuptaSubir Sarker - La Trobe University
- Publication Details
- Viruses, Vol.17(8), 1126
- Publisher
- MDPI
- Number of pages
- 15
- Identifiers
- 991005810049907891
- Copyright
- © 2025 by the authors.
- Murdoch Affiliation
- Harry Butler Institute; School of Veterinary Medicine; Centre for Animal Production and Health
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.1662 Poxvirus Immunology
- Web Of Science research areas
- Virology
- ESI research areas
- Microbiology