Journal article
Integrative modeling of plasma metabolic and lipoprotein biomarkers of SARS-CoV-2 infection in Spanish and Australian COVID-19 patient cohorts
Journal of Proteome Research, Vol.20(8), pp.4139-4152
2021
Abstract
Quantitative plasma lipoprotein and metabolite profiles were measured on an autonomous community of the Basque Country (Spain) cohort consisting of hospitalized COVID-19 patients (n = 72) and a matched control group (n = 75) and a Western Australian (WA) cohort consisting of (n = 17) SARS-CoV-2 positives and (n = 20) healthy controls using 600 MHz 1H nuclear magnetic resonance (NMR) spectroscopy. Spanish samples were measured in two laboratories using one-dimensional (1D) solvent-suppressed and T2-filtered methods with in vitro diagnostic quantification of lipoproteins and metabolites. SARS-CoV-2 positive patients and healthy controls from both populations were modeled and cross-projected to estimate the biological similarities and validate biomarkers. Using the top 15 most discriminatory variables enabled construction of a cross-predictive model with 100% sensitivity and specificity (within populations) and 100% sensitivity and 82% specificity (between populations). Minor differences were observed between the control metabolic variables in the two cohorts, but the lipoproteins were virtually indistinguishable. We observed highly significant infection-related reductions in high-density lipoprotein (HDL) subfraction 4 phospholipids, apolipoproteins A1 and A2,that have previously been associated with negative regulation of blood coagulation and fibrinolysis. The Spanish and Australian diagnostic SARS-CoV-2 biomarkers were mathematically and biologically equivalent, demonstrating that NMR-based technologies are suitable for the study of the comparative pathology of COVID-19 via plasma phenotyping.
Details
- Title
- Integrative modeling of plasma metabolic and lipoprotein biomarkers of SARS-CoV-2 infection in Spanish and Australian COVID-19 patient cohorts
- Authors/Creators
- R. Masuda (Author/Creator) - Murdoch UniversityS. Lodge (Author/Creator) - Murdoch UniversityP. Nitschke (Author/Creator) - Murdoch UniversityM. Spraul (Author/Creator) - Bruker (United States)H. Schaefer (Author/Creator) - Bruker (United States)S-H Bong (Author/Creator) - Murdoch UniversityT. Kimhofer (Author/Creator) - Murdoch UniversityD. Hall (Author/Creator) - Murdoch UniversityR.L. Loo (Author/Creator) - Murdoch UniversityM. Bizkarguenaga (Author/Creator) - CIC bioGUNEC. Bruzzone (Author/Creator) - CIC bioGUNER. Gil-Redondo (Author/Creator) - CIC bioGUNEN. Embade (Author/Creator) - CIC bioGUNEJ.M. Mato (Author/Creator) - CIC bioGUNEE. Holmes (Author/Creator) - Murdoch UniversityJ. Wist (Author/Creator) - Murdoch UniversityO. Millet (Author/Creator) - CIC bioGUNEJ.K. Nicholson (Author/Creator) - Murdoch University
- Publication Details
- Journal of Proteome Research, Vol.20(8), pp.4139-4152
- Publisher
- American Chemical Society
- Identifiers
- 991005542462407891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Australian National Phenome Centre; Centre for Computational and Systems Medicine; Health Futures Institute
- Language
- English
- Resource Type
- Journal article
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- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
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