Journal article
Interferon-Gamma responses to candida recover slowly or remain low in immunodeficient HIV patients responding to ART
Journal of Clinical Immunology, Vol.26(2), pp.160-167
2006
Abstract
Extended assessments of memory T-cell responses in HIV patients who have a satisfactory virological response to combination antiretroviral therapy (CART) have been limited by availability of longitudinal samples and of antigens to which most individuals (including HIV-negative controls) have been exposed. Studies of cytomegalovirus (CMV) show that interferon-gamma (IFN-;3) responses never recover completely, but this may be antigen-specific. Here we present responses to Candida and CMV antigens analyzed using a statistical approach that derives overall trends from samples collected at variable time points. Results were considered in relation to putative markers of T-regulatory cells. Blood mononuclear cells collected from seventeen HIV-1 patients (nadir <100 CD4 T cells/mL) 0 138 years after initiation of CART were stimulated with Candida spp lysate, Candida enolase protein or CMV lysate and production of IFN-;3 was assessed by ELISpot assay. CD4 T-cell counts increased fivefold and stabilized within 24 months on CART, following control of plasma viremia. IFN-;3 responses to Candida antigens began low and increased slowly, generating positive slope up to 60 months on CART (Candida enolase p=0.008; Candida lysate p=0.03; mixed-model Wald test). Only two patients displayed a CMV or Candida-specific IFN-;3 response above the median for seronegative controls. Proportions of T cells expressing CD25 or CD57 did not correlate with IFN-;3 responses. Slow reconstitution of IFN-;3 responses to CMV and Candida in previously immunodeficient patients with restored CD4+ T-cell counts on CART suggests a broad and nonresolving defect in memory T-cell responses.
Details
- Title
- Interferon-Gamma responses to candida recover slowly or remain low in immunodeficient HIV patients responding to ART
- Authors/Creators
- K. Burgess (Author/Creator) - The University of Western AustraliaP. Price (Author/Creator) - Royal Perth HospitalI.R. James (Author/Creator) - Royal Perth HospitalS.F. Stone (Author/Creator) - The University of Western AustraliaN.M. Keane (Author/Creator) - Royal Perth HospitalA.Y.F. Lim (Author/Creator) - Royal Perth HospitalJ.R. Warmington (Author/Creator) - Curtin UniversityM.A. French (Author/Creator) - Royal Perth Hospital
- Publication Details
- Journal of Clinical Immunology, Vol.26(2), pp.160-167
- Publisher
- Springer US
- Identifiers
- 991005542657107891
- Copyright
- 2006 Springer Science + Business Media, Inc.
- Murdoch Affiliation
- Centre for Clinical Immunology and Biomedical Statistics
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
44 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.66 HIV
- 1.66.46 HIV Pathogenesis
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology