Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs.

M.A. Stanley; R.A. Moore; P.K. Nicholls; E.B. Santos; L. Thomsen; N. Parry; S. Walcott; G. Gough

doi:10.1016/S0264-410X(00)00533-8

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Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs.

Journal article

Open access

Peer reviewed

Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs.

M.A. Stanley, R.A. Moore, P.K. Nicholls, E.B. Santos, L. Thomsen, N. Parry, S. Walcott and G. Gough

Vaccine, Vol.19(20-22), pp.2783-2792

2001

DOI: https://doi.org/10.1016/S0264-410X(00)00533-8

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Abstract

Protection against viral challenge with canine oral papillomavirus (COPV) was achieved by immunisation via particle-mediated DNA delivery (PMDD) of a plasmid encoding the COPV L1 gene to cutaneous and oral mucosal sites in beagle dogs. The initial dose of approximately 9 μg of DNA was followed by two booster doses at 6 week intervals. A similar approach was used to vaccinate a control group of animals with plasmid DNA encoding the Hepatitis B virus S gene. Following challenge at the oral mucosa with COPV all animals vaccinated with the COPV L1 gene were protected against disease. However five of six animals in the control group developed COPV induced papillomas at the oral mucosa. Both cell-mediated lymphoproliferative and humoral antibody responses to the DNA vaccine were observed. Our data indicate that PMDD of plasmid DNA can protect against mucosal challenge with papillomavirus.

Details

Title: Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs.
Authors/Creators: M.A. Stanley (Author/Creator)
R.A. Moore (Author/Creator)
P.K. Nicholls (Author/Creator)
E.B. Santos (Author/Creator)
L. Thomsen (Author/Creator)
N. Parry (Author/Creator)
S. Walcott (Author/Creator)
G. Gough (Author/Creator)
Publication Details: Vaccine, Vol.19(20-22), pp.2783-2792
Publisher: Elsevier BV
Identifiers: 991005541638807891
Copyright: © 2001 Elsevier Science Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.179 Oncology; 1.179.108 HPV and Cervical Cancer
Web Of Science research areas: Immunology; Medicine, Research & Experimental
ESI research areas: Immunology