Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

K.M. Morabito; T.R. Ruckwardt; A.J. Redwood; S.M. Moin; D.A. Price; B.S. Graham

doi:10.1038/mi.2016.48

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Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

Journal article

Open access

Peer reviewed

Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung

K.M. Morabito, T.R. Ruckwardt, A.J. Redwood, S.M. Moin, D.A. Price and B.S. Graham

Mucosal Immunology, Vol.10, pp.545-554

2016

DOI: https://doi.org/10.1038/mi.2016.48

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Abstract

Cytomegalovirus vectors are promising delivery vehicles for vaccine strategies that aim to elicit effector CD8+ T cells. To determine how the route of immunization affects CD8+ T cell responses in the lungs of mice vaccinated with a murine cytomegalovirus vector expressing the respiratory syncytial virus matrix (M) protein, we infected CB6F1 mice via the intranasal or intraperitoneal route and evaluated the M-specific CD8+ T cell response at early and late time points. We found that intranasal vaccination generated robust and durable tissue-resident effector and effector memory CD8+ T cell populations that were undetectable after intraperitoneal vaccination. The generation of these antigen-experienced cells by intranasal vaccination resulted in earlier T cell responses, interferon gamma secretion, and viral clearance after respiratory syncytial virus challenge. Collectively, these findings validate a novel approach to vaccination that emphasizes the route of delivery as a key determinant of immune priming at the site of vulnerability.

Details

Title: Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung
Authors/Creators: K.M. Morabito (Author/Creator) - National Institute of Allergy and Infectious Diseases
T.R. Ruckwardt (Author/Creator) - National Institute of Allergy and Infectious Diseases
A.J. Redwood (Author/Creator) - Murdoch University
S.M. Moin (Author/Creator) - National Institute of Allergy and Infectious Diseases
D.A. Price (Author/Creator) - Cardiff University
B.S. Graham (Author/Creator) - National Institute of Allergy and Infectious Diseases
Publication Details: Mucosal Immunology, Vol.10, pp.545-554
Publisher: Nature Publishing Group
Identifiers: 991005544967707891
Copyright: © 2016 Society for Mucosal Immunology
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.6 Immunology; 1.6.127 T Cell Regulation
Web Of Science research areas: Immunology
ESI research areas: Immunology