Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility

K. Kaewmala; M.J. Uddin; M.U. Çınar; C. Große-Brinkhaus; E. Jonas; D. Tesfaye; C. Phatsara; E. Tholen; C. Looft; K. Schellander

doi:10.1111/j.1439-0531.2011.01831.x

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Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility

Journal article

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Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility

K. Kaewmala, M.J. Uddin, M.U. Çınar, C. Große-Brinkhaus, E. Jonas, D. Tesfaye, C. Phatsara, E. Tholen, C. Looft and K. Schellander

Reproduction in Domestic Animals, Vol.47(2), pp.213-223

2011

DOI: https://doi.org/10.1111/j.1439-0531.2011.01831.x

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Abstract

Phospholipase C zeta (PLCz) and cyclooxygenase isoenzyme type 2 (COX-2) are important in spermatogenesis, but their effect has not yet confirmed in pigs. Therefore, this study was aimed to analyse their association with sperm quality and fertility and to identify the mRNA and protein expression in boars reproductive tissues. DNA samples from 231 Pietrain (PI) and 109 Pietrain × Hampshire (PIHA) pigs with records of sperm quality [sperm concentration (SCON), motility, semen volume, plasma droplet and abnormal spermatozoa rate] and fertility (non-return rate and number of piglet born alive) traits were available. A SNP in non-coding region of PLCz g.158 A > C was associated with SCON (p < 0.05) in PIHA population while the polymorphism of COX-2 g.68 G > A in 3′ UTR was not associated with any traits. For mRNA and protein expression study, a total of six boars were divided into two groups with G-I and G-II, where G-I was characterized for relatively better sperm quality. Both genes expressed higher in reproductive tissues compared with non-reproductive tissues. Phospholipase C zeta mRNA expressed higher in testis (p < 0.01), all parts of epididymis and spermatozoa from G-I, while COX-2 expressed higher in testis (p < 0.05), head and body of epididymis (p < 0.01), and spermatozoa from G-II boar. Both proteins were localized in Leydig cells and spermatozoa. These results might shed light on roles of these genes in spermatogenesis as candidate for boar sperm quality and fertility, but still the lack of association across populations should be considered.

Details

Title: Investigation into Association and Expression of PLCz and COX-2 as Candidate Genes for Boar Sperm Quality and Fertility
Authors/Creators: K. Kaewmala (Author/Creator)
M.J. Uddin (Author/Creator)
M.U. Çınar (Author/Creator)
C. Große-Brinkhaus (Author/Creator)
E. Jonas (Author/Creator)
D. Tesfaye (Author/Creator)
C. Phatsara (Author/Creator)
E. Tholen (Author/Creator)
C. Looft (Author/Creator)
K. Schellander (Author/Creator)
Publication Details: Reproduction in Domestic Animals, Vol.47(2), pp.213-223
Publisher: Blackwell Publishing Inc.
Identifiers: 991005543148107891
Copyright: © 2011 Blackwell Verlag GmbH
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.339 Embryo Development
Web Of Science research areas: Agriculture, Dairy & Animal Science; Reproductive Biology; Veterinary Sciences
ESI research areas: Agricultural Sciences