Investigation of the morphological diversity of the potentially zoonotic Trypanosoma copemani in quokkas and Gilbert's potoroos

J.M. Austen; S.A. Reid; D.R. Robinson; J.A. Friend; W.G.F. Ditcham; P.J. Irwin; U. Ryan

doi:10.1017/S0031182015000785

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Investigation of the morphological diversity of the potentially zoonotic Trypanosoma copemani in quokkas and Gilbert's potoroos

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Investigation of the morphological diversity of the potentially zoonotic Trypanosoma copemani in quokkas and Gilbert's potoroos

J.M. Austen, S.A. Reid, D.R. Robinson, J.A. Friend, W.G.F. Ditcham, P.J. Irwin and U. Ryan

Parasitology, Vol.142(11), pp.1443-1452

2015

DOI: https://doi.org/10.1017/S0031182015000785

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Abstract

Trypanosomes are blood-borne parasites that can cause severe disease in both humans and animals, yet little is known of the pathogenicity and life-cycles of trypanosomes in native Australian mammals. Trypanosoma copemani is known to be infective to a variety of Australian marsupials and has recently been shown to be potentially zoonotic as it is resistant to normal human serum. In the present study, in vivo and in vitro examination of blood and cultures from Australian marsupials was conducted using light microscopy, immunofluorescence, scanning electron microscopy and fluorescence in situ hybridization. Promastigote, sphaeromastigote and amastigote life-cycle stages were detected in vivo and in vitro. Novel trypanosome-like stages were also detected both in vivo and in vitro representing an oval stage, an extremely thin stage, an adherent stage and a tiny round stage. The tiny round and adherent stages appeared to adhere to erythrocytes causing potential haematological damage with clinical effects similar to haemolytic anaemia. The present study shows for the first time that trypomastigotes are not the only life-cycle stages circulating within the blood stream of trypanosome infected Australian native marsupials and provides insights into possible pathogenic mechanisms of this potentially zoonotic trypanosome species.

Details

Title: Investigation of the morphological diversity of the potentially zoonotic Trypanosoma copemani in quokkas and Gilbert's potoroos
Authors/Creators: J.M. Austen (Author/Creator) - Murdoch University
S.A. Reid (Author/Creator) - The University of Queensland
D.R. Robinson (Author/Creator) - Université de Bordeaux
J.A. Friend (Author/Creator) - Department of Parks and Wildlife
W.G.F. Ditcham (Author/Creator)
P.J. Irwin (Author/Creator) - Murdoch University
U. Ryan (Author/Creator) - Murdoch University
Publication Details: Parasitology, Vol.142(11), pp.1443-1452
Publisher: Cambridge University Press
Identifiers: 991005540044407891
Copyright: © Cambridge University Press 2015
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.261 Parasitology - Trypanosoma & Leishmania; 1.261.596 Trypanosoma Biology
Web Of Science research areas: Parasitology
ESI research areas: Microbiology