Journal article
Investigation of twisted intercalating nucleic acid (TINA)-modified antisense oligonucleotides for splice modulation by induced exon-skipping in vitro
RSC Advances, Vol.6(97), pp.95169-95172
2016
Abstract
Twisted intercalating nucleic acids (TINA)-modified oligonucleotides containing phenylethynylpyren-1-yl derivatives were reported to improve the thermal stability of the nucleic acid duplexes, triplexes and G-quadruplexes. In the present study, we have investigated the potential of TINA-modified antisense oligonucleotides (AOs) in splice modulation by induced exon-skipping in vitro. We used both para-TINA (p-TINA) and ortho-TINA (o-TINA)-modified 2′-O-methyl (2′-OMe) AOs on a phosphorothioate backbone (PS) designed to skip exon-23 in dystrophin pre-mRNA transcript in mdx mice myotubes. A fully-modified 2′-OMePS AO control was used in parallel. Our results showed that both p-TINA and o-TINA-modified AOs were able to induce exon-23 skipping. These results provide new insights on expanding the applicability of TINA-modified oligonucleotides.
Details
- Title
- Investigation of twisted intercalating nucleic acid (TINA)-modified antisense oligonucleotides for splice modulation by induced exon-skipping in vitro
- Authors/Creators
- B.T. Le (Author/Creator) - Murdoch UniversityV.V. Filichev (Author/Creator) - Massey UniversityR.N. Veedu (Author/Creator) - Murdoch University
- Publication Details
- RSC Advances, Vol.6(97), pp.95169-95172
- Publisher
- Royal Society of Chemistry
- Identifiers
- 991005540086807891
- Copyright
- © 2016 Royal Society of Chemistry
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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