Journal article
Iron overload impairs pro-inflammatory cytokine responses by Kupffer cells
Journal of Gastroenterology and Hepatology, Vol.16(4), pp.438-444
2001
Abstract
Aim: The aim of the present study was to determine the effect of chronic iron overload on Kupffer cell cytokine production.
Methods: Kupffer cells were isolated from rats that were fed either a control or iron-supplemented diet for 12 months. Cytokine mRNA and protein levels were determined by using a ribonuclease protection assay and ELISA, respectively.
Results: Baseline levels of tumor necrosis factor-α, transforming growth factor-β1, interleukin-6 and granulocyte macrophage colony stimulating factor were similar in iron-loaded and control Kupffer cells. Following the addition of lipopolysaccharide to control cells, tumor necrosis factor-alpha, interleukin-1α and interleukin-6 mRNA levels increased. Tumor necrosis factor-alpha mRNA and protein levels were reduced by 40 and 60%, respectively, in iron-loaded cells compared with controls following the addition of lipopolysaccharide. Interleukin-6 mRNA levels in iron-loaded Kupffer cells were also reduced. Granulocyte macrophage colony stimulating factor mRNA levels remained unchanged in controls, but were significantly elevated in iron-loaded cells. Tumor growth factor-β1 mRNA and protein levels were similar in control and iron-loaded cells.
Conclusion: Deposition of iron in Kupffer cells in chronic dietary iron overload results in an impaired pro-inflammatory cytokine response to lipopolysaccharide. Our observations may have relevance to the altered immune function observed in chronic iron-overload syndromes.
Details
- Title
- Iron overload impairs pro-inflammatory cytokine responses by Kupffer cells
- Authors/Creators
- J.K. Olynyk (Author/Creator)S.L. Clarke (Author/Creator)
- Publication Details
- Journal of Gastroenterology and Hepatology, Vol.16(4), pp.438-444
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005541691307891
- Copyright
- © 2001 Blackwell Science Asia Pty Ltd
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.184 Physiology & Metals
- 1.184.573 Iron Metabolism
- Web Of Science research areas
- Gastroenterology & Hepatology
- ESI research areas
- Clinical Medicine