Isolation and Characterization of Microsatellite Primers for the Critically Endangered Shrub Styphelia longissima (Ericaceae)

Janet M Anthony; Richard J. N Allcock; Mark P Dobrowolski; Siegfried L Krauss

doi:10.3732/apps.1700108

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Isolation and Characterization of Microsatellite Primers for the Critically Endangered Shrub Styphelia longissima (Ericaceae)

Journal article

Open access

Peer reviewed

Isolation and Characterization of Microsatellite Primers for the Critically Endangered Shrub Styphelia longissima (Ericaceae)

Janet M Anthony, Richard J. N Allcock, Mark P Dobrowolski and Siegfried L Krauss

Applications in plant sciences, Vol.5(11), 1700108

2017

DOI: https://doi.org/10.3732/apps.1700108

PMCID: PMC5703184

PMID: 29188149

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Abstract

Astroloma xerophyllum

Ericaceae

microsatellite primers

PRIMER NOTES

shotgun sequencing

Styphelia longissima

Premise of the study: Microsatellite markers were developed for population genetic analysis in the rare shrub Styphelia longissima (Ericaceae). Methods and Results: We generated ca. 2.5 million sequence reads using a Personal Genome Machine semiconductor sequencer. Using the QDD pipeline, we designed primers for >12,000 sequences with PCR product lengths of 80–480 bp. From these, 30 primer pairs were selected and screened using PCR; of these, 16 loci were found to be polymorphic, four loci were monomorphic, and 10 loci did not amplify reliably for S. longissima. For a sample of 57 plants from the only known population, the number of alleles observed for these 16 loci ranged from two to 21 and expected heterozygosity ranged from 0.49 to 0.91. These markers were also amplified in Astroloma xerophyllum, a closely related species. Conclusions: These markers will be used to characterize population genetic variation, spatial genetic structure, mating system parameters, and dispersal to aid in the management and conservation of the rare shrub S. longissima.

Details

Title: Isolation and Characterization of Microsatellite Primers for the Critically Endangered Shrub Styphelia longissima (Ericaceae)
Authors/Creators: Janet M Anthony - The University of Western Australia
Richard J. N Allcock - The University of Western Australia
Mark P Dobrowolski - Level
Siegfried L Krauss - The University of Western Australia
Publication Details: Applications in plant sciences, Vol.5(11), 1700108
Publisher: Botanical Society of America
Number of pages: 3
Identifiers: 991005579911307891
Murdoch Affiliation: Centre for Terrestrial Ecosystem Science and Sustainability
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.64 Phylogenetics & Genomics; 3.64.71 Genetic Diversity
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science