Isolation and characterisation of 36 polymorphic microsatellite markers using 454 sequencing in the bar-shouldered skink, Ctenotus inornatus

E. Harradine; R.A. How; L.H. Schmitt; C. de Hass; P.B.S. Spencer

doi:10.1007/s12686-012-9769-4

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Isolation and characterisation of 36 polymorphic microsatellite markers using 454 sequencing in the bar-shouldered skink, Ctenotus inornatus

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Isolation and characterisation of 36 polymorphic microsatellite markers using 454 sequencing in the bar-shouldered skink, Ctenotus inornatus

E. Harradine, R.A. How, L.H. Schmitt, C. de Hass and P.B.S. Spencer

Conservation Genetics Resources, Vol.5(1), pp.207-210

2013

DOI: https://doi.org/10.1007/s12686-012-9769-4

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Abstract

We report on the development of 36 novel polymorphic microsatellite markers from the bar-shouldered skink, Ctenotus inornatus. Members in this genus account for up to 20 % of the diverse Australian lizard species. Microsatellite markers were developed to investigate island endemism amongst Kimberley islands taxa. We describe the diversity from 110 adult Ctenotus individuals. Overall, the markers produced between 9 to 40 alleles per locus and observed and expected heterozygosities of 74. 6 and 95. 7 %, respectively. Due to island endemism, habitat modification by introduced grazing mammals, some urban encroachment and changes in fire regime there has been fragmentation of its range. These microsatellite markers are an important tool for evaluating population genetic information and for prioritising populations for conservation management.

Details

Title: Isolation and characterisation of 36 polymorphic microsatellite markers using 454 sequencing in the bar-shouldered skink, Ctenotus inornatus
Authors/Creators: E. Harradine (Author/Creator) - Murdoch University
R.A. How (Author/Creator) - Western Australian Museum
L.H. Schmitt (Author/Creator) - The University of Western Australia
C. de Hass (Author/Creator) - The University of Western Australia
P.B.S. Spencer (Author/Creator)
Publication Details: Conservation Genetics Resources, Vol.5(1), pp.207-210
Publisher: Springer Verlag
Identifiers: 991005543416507891
Murdoch Affiliation: School of Biological Sciences and Biotechnology
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.64 Phylogenetics & Genomics; 3.64.71 Genetic Diversity
Web Of Science research areas: Biodiversity Conservation; Genetics & Heredity
ESI research areas: Environment/Ecology