Journal article
Isolation and characterization of putative Pseudobutyrivibrio ruminis promoters
Microbiology, Vol.153(9), pp.3071-3080
09/2007
Abstract
Novel plasmids were constructed for the analysis of DNA fragments from the rumen bacterium Pseudobutyrivibrio ruminis. Five previously unidentified promoters were characterized using a novel primer extension method to identify transcription start sites. The genes downstream of these promoters were not identified, and their activity in expression of genomic traits in wild-type P. ruminis remains putative. Comparison with promoters from this and closely related species revealed a consensus sequence resembling the binding motif for the RNA polymerase σ 70-like factor complex. Consensus -35 and -10 sequences within these elements were TTGACA and ATAATATA respectively, interspaced by 15-16 bp. The consensus for the -10 element was extended by one nucleotide upstream and downstream of the standard hexamer (indicated in bold). Promoter strengths were measured by reverse transcription quantitative PCR and β-glucuronidase assays. No correlation was found between the composition and context of elements within P. ruminis promoters, and promoter strength. However, a mutation within the -35 element of one promoter revealed that transcriptional strength and choice of transcription start site were sensitive to this single nucleotide change.
Details
- Title
- Isolation and characterization of putative Pseudobutyrivibrio ruminis promoters
- Authors/Creators
- T.D. Schoep (Author/Creator)K. Gregg (Author/Creator)
- Publication Details
- Microbiology, Vol.153(9), pp.3071-3080
- Publisher
- Society for General Microbiology
- Identifiers
- 991005545358807891
- Copyright
- © 2007 SGM.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- 1.42 Bacteriology
- 1.42.131 Bacterial Gene Regulation
- Web Of Science research areas
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- Microbiology