Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis

W.K. Greene; W.J. Penhale; R.C.A. Thompson

doi:10.1016/0165-2427(93)90012-S

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Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis

Journal article

Peer reviewed

Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis

W.K. Greene, W.J. Penhale and R.C.A. Thompson

Veterinary Immunology and Immunopathology, Vol.37(1), pp.15-23

1993

DOI: https://doi.org/10.1016/0165-2427(93)90012-S

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Abstract

Polyadenylated [poly(A)+] mRNA was isolated from the cat flea, Ctenocephalides felis by oligothymidylic acid-cellulose spin column chromatography and translated in vitro using a cell-free rabbit reticulocyte system. The relative incorporation of 35S-methionine into trichloroacetic acid-precipitable translation products obtained using poly (A+) mRNA was 48.5-fold over control translations performed without added mRNA. SDS-PAGE analysis of the translation products in combination with autoradiography showed that many proteins with apparent molecular weights in the range 14-90 K were synthesized. Immunoprecipitation studies performed using flea-allergic dog sera showed that several of the synthesized proteins corresponded with flea allergens. The allergenicity of the lysates was also confirmed by skin testing.

Details

Title: Isolation and in vitro translation of messenger RNA encoding allergens of the cat flea, Ctenocephalides felis
Authors/Creators: W.K. Greene (Author/Creator) - Murdoch University
W.J. Penhale (Author/Creator) - Murdoch University
R.C.A. Thompson (Author/Creator) - Murdoch University
Publication Details: Veterinary Immunology and Immunopathology, Vol.37(1), pp.15-23
Publisher: Elsevier BV
Identifiers: 991005544955707891
Copyright: © Elsevier B.V.
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.65 Allergy; 1.65.264 Allergy Mechanisms
Web Of Science research areas: Immunology; Veterinary Sciences
ESI research areas: Plant & Animal Science