Journal article
Isolation and primary culture of rat Kupffer cells
Journal of Gastroenterology and Hepatology, Vol.13(8), pp.843-845
1998
Abstract
The aim of this study was to describe a reproducible method for the isolation, purification and primary culture of rat Kupffer cells. Kupffer cells were isolated following sequential pronase/collagenase digestion of the liver and enrichment of a non-parenchymal cell fraction by a single-density gradient centrifugation step using 30% metrizamide. Kupffer cells were isolated and further purified from this cell fraction by centrifugal elutriation. Kupffer cells were isolated at 1017 g at 48–110 mL/min. All Kupffer cell fractions exhibited phagocytosis of 3 μm latex beads. Kupffer cell fractions isolated at 48 and 60 mL/min were predominantly ED2 negative while later fractions (80–110 mL/min) were ED2 positive. Kupffer cells were adherent in culture after 2 h. This method for Kupffer cell isolation resulted in a yield of 80–120 times 106 Kupffer cells per liver.
Details
- Title
- Isolation and primary culture of rat Kupffer cells
- Authors/Creators
- J.K. Olynyk (Author/Creator) - Fremantle HospitalS.L. Clarke (Author/Creator) - Fremantle Hospital
- Publication Details
- Journal of Gastroenterology and Hepatology, Vol.13(8), pp.843-845
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005544407907891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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