Journal article
Isolation and sequence analysis of DREB2A homologues in three cereal and two legume species
Plant Science, Vol.177(5), pp.460-467
2009
Abstract
The transcription factor, DREB2A, is one of the promising candidate genes involved in dehydration tolerance in crop plants. In order to isolate DREB2A homologues across cereals (rice, barley and sorghum) and legumes (common bean and chickpea), specific or degenerate primers were used. Gene/phylogenetic trees were constructed using a non-redundant set of 19 DREB1A and 27 DREB2A amino acid sequences and were combined with taxonomic/species tree to prepare reconciled phylogenetic trees. In total, 86 degenerate primers were designed for different clades and 295 degenerate primer combinations were used to amplify DREB homologues in targeted crop species. Successful amplification of DREB2A was obtained in case of sorghum. In parallel, gene-specific primers were used to amplify DREB2A homologues in rice, barley, common bean and chickpea. Seven to eight diverse genotypes from targeted species were used for sequence analysis at DREB2A locus identified/isolated. A maximum of eight SNPs were found in the common bean DREB2A, indicating two distinct haplotypes, three SNPs with five haplotypes were observed in barley whereas a single SNP was observed in rice, sorghum and chickpea. Parsimony based phylogenetic tree revealed distinct clustering of cereals and legumes. Furthermore, alignment of corresponding amino acid sequences showed conservation of AP2 domain across the targeted species.
Details
- Title
- Isolation and sequence analysis of DREB2A homologues in three cereal and two legume species
- Authors/Creators
- S.N. Nayak (Author/Creator) - Osmania UniversityJ. Balaji (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.D. Upadhyaya (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsC.T. Hash (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsP.B.K. Kishor (Author/Creator) - Osmania UniversityD. Chattopadhyay (Author/Creator) - National Institute of Plant Genome ResearchL.M. Rodriquez (Author/Creator) - International Center for Tropical AgricultureM.W. Blair (Author/Creator) - International Center for Tropical AgricultureM. Baum (Author/Creator) - International Center for Agricultural Research in the Dry AreasK. McNally (Author/Creator) - International Rice Research InstituteD. This (Author/Creator) - SupAgroD.A. Hoisington (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsR.K. Varshney (Author/Creator) - Centro Internacional de Mejoramiento de Maíz Y Trigo
- Publication Details
- Plant Science, Vol.177(5), pp.460-467
- Publisher
- Elsevier
- Identifiers
- 991005545075207891
- Copyright
- © 2009 Elsevier Ireland Ltd.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.49 Plant Stress Responses
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science