Journal article
Isolation of Oscillatoria spongeliae, the filamentous cyanobacterial symbiont of the marine sponge Dysidea herbacea
Marine Biology, Vol.119(1), pp.99-104
1994
Abstract
The tropical marine sponge Dysidea herbacea (Keller) (Dictyoceratidae: Dysideidae) is always found associated with the filamentous cyanobacterium (blue-green alga) Oscillatoria spongeliae (Schulze) Hauck (Cyanophyceae: Oscillatoriaceae), which occurs abundantly throughout the sponge mesohyl. Intact, metabolically active, trichomes of O. spongeliae were isolated from the sponge by chopping the sponge tissue with a razor blade and squeezing the trichomes into a seawater-based medium containing polyvinylpyrrolidone, bovine serum albumin, dithiothreitol, glycerol, KCl and Na2CO3. The isolated cyanobacteria were concentrated by centrifugation and then washed several times in fresh medium. The isolated O. spongeliae have photosynthetic rates which are similar to the intact sponge-alga association for periods of at least 6 h after isolation. Addition of sponge homogenate to the isolated cyanobacteria causes rapid cell lysis.
Details
- Title
- Isolation of Oscillatoria spongeliae, the filamentous cyanobacterial symbiont of the marine sponge Dysidea herbacea
- Authors/Creators
- R. Hinde (Author/Creator) - The University of SydneyF. Pironet (Author/Creator) - The University of SydneyM.A. Borowitzka (Author/Creator) - Murdoch University
- Publication Details
- Marine Biology, Vol.119(1), pp.99-104
- Publisher
- Springer Verlag
- Identifiers
- 991005540987807891
- Copyright
- © 1994 Springer-Verlag.
- Murdoch Affiliation
- School of Biological and Environmental Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
29 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.198 Mycotoxins
- 3.198.749 Porifera
- Web Of Science research areas
- Marine & Freshwater Biology
- ESI research areas
- Plant & Animal Science