Isolation of the Pandemic (H1N1) 2009 virus and its reassortant with an H3N2 swine influenza virus from healthy weaning pigs in Thailand in 2011

Y. Hiromoto; S. Parchariyanon; N. Ketusing; P. Netrabukkana; T. Hayashi; T. Kobayashi; N. Takemae; T. Saito

doi:10.1016/j.virusres.2012.07.025

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Isolation of the Pandemic (H1N1) 2009 virus and its reassortant with an H3N2 swine influenza virus from healthy weaning pigs in Thailand in 2011

Journal article

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Isolation of the Pandemic (H1N1) 2009 virus and its reassortant with an H3N2 swine influenza virus from healthy weaning pigs in Thailand in 2011

Y. Hiromoto, S. Parchariyanon, N. Ketusing, P. Netrabukkana, T. Hayashi, T. Kobayashi, N. Takemae and T. Saito

Virus Research, Vol.169(1), pp.175-181

2012

DOI: https://doi.org/10.1016/j.virusres.2012.07.025

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Abstract

A total of 300 nasal swabs were collected from 5 pig farms in two provinces in the Eastern part of Thailand in February 2011 and were subjected to viral isolation of influenza A viruses. Two H3N2 and 6 H1N1 influenza A viruses were isolated from swabs collected from clinically healthy weaning pigs on farms in Chonburi and Chachoengsao provinces, respectively. The H3N2 isolates consisted of the hemagglutinin (HA) and neuraminidase (NA) genes closely related to Thai SIVs and derived from a cluster of human seasonal H3N2 strains circulating around 1996-1997. The remaining gene segments of the isolates originated from the Pandemic (H1N1) 2009 (A (H1N1) pdm09) virus. Antigenicity of the H3N2 isolates was distinguishable from a human seasonal vaccine strain in the 1996-1998 seasons that represented antigenicity of the seasonal strains around 1996-1998. Nasal swabs from a Chachoengsao farm yielded A (H1N1) pdm09 viruses in chicken embryonated eggs and MDCK cells. A (H1N1) pdm09 viruses isolated in this study grew poorly in MDCK cells. Deduced amino acid sequences of the HA1 region of the HA protein of egg isolated viruses were identical to the sequences directly amplified from original swab samples. Our result demonstrated that the A (H1N1) pdm09 virus has been established in the Thai pig population and this has resulted in genetic reassortment with Thai SIV that previously circulated among pigs.

Details

Title: Isolation of the Pandemic (H1N1) 2009 virus and its reassortant with an H3N2 swine influenza virus from healthy weaning pigs in Thailand in 2011
Authors/Creators: Y. Hiromoto (Author/Creator) - National Agriculture and Food Research Organization
S. Parchariyanon (Author/Creator) - National Institute of Animal Health
N. Ketusing (Author/Creator) - National Institute of Animal Health
P. Netrabukkana (Author/Creator) - Murdoch University
T. Hayashi (Author/Creator) - National Agriculture and Food Research Organization
T. Kobayashi (Author/Creator) - National Agriculture and Food Research Organization
N. Takemae (Author/Creator) - National Agriculture and Food Research Organization
T. Saito (Author/Creator) - National Agriculture and Food Research Organization
Publication Details: Virus Research, Vol.169(1), pp.175-181
Publisher: Elsevier BV
Identifiers: 991005542904807891
Copyright: © 2012 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.104 Virology - General; 1.104.126 Influenza
Web Of Science research areas: Virology
ESI research areas: Microbiology