Journal article
Karyotype differentiation in cultivated chickpea revealed by Oligopainting fluorescence in situ hybridization
Frontiers in Plant Science, Vol.12, Art. 791303
2022
Abstract
Chickpea (Cicer arietinum L.) is one of the main sources of plant proteins in the Indian subcontinent and West Asia, where two different morphotypes, desi and kabuli, are grown. Despite the progress in genome mapping and sequencing, the knowledge of the chickpea genome at the chromosomal level, including the long-range molecular chromosome organization, is limited. Earlier cytogenetic studies in chickpea suffered from a limited number of cytogenetic landmarks and did not permit to identify individual chromosomes in the metaphase spreads or to anchor pseudomolecules to chromosomes in situ. In this study, we developed a system for fast molecular karyotyping for both morphotypes of cultivated chickpea. We demonstrate that even draft genome sequences are adequate to develop oligo-fluorescence in situ hybridization (FISH) barcodes for the identification of chromosomes and comparative analysis among closely related chickpea genotypes. Our results show the potential of oligo-FISH barcoding for the identification of structural changes in chromosomes, which accompanied genome diversification among chickpea cultivars. Moreover, oligo-FISH barcoding in chickpea pointed out some problematic, most probably wrongly assembled regions of the pseudomolecules of both kabuli and desi reference genomes. Thus, oligo-FISH appears as a powerful tool not only for comparative karyotyping but also for the validation of genome assemblies.
Details
- Title
- Karyotype differentiation in cultivated chickpea revealed by Oligopainting fluorescence in situ hybridization
- Authors/Creators
- A. Doležalová (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyL. Sládeková (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyD. Šimoníková (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyK. Holušová (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyM. Karafiátová (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsJ. Doležel (Author/Creator) - Czech Academy of Sciences, Institute of Experimental BotanyE. Hřibová (Author/Creator) - Czech Academy of Sciences, Institute of Experimental Botany
- Publication Details
- Frontiers in Plant Science, Vol.12, Art. 791303
- Publisher
- Frontiers
- Identifiers
- 991005543286007891
- Copyright
- © 2022 Doležalová et al.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; Food Futures Institute; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science