Journal article
Key regulators of sucrose metabolism identified through comprehensive comparative transcriptome analysis in peanuts
International Journal of Molecular Sciences, Vol.22(14), Art. 7266
2021
Abstract
Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts.
Details
- Title
- Key regulators of sucrose metabolism identified through comprehensive comparative transcriptome analysis in peanuts
- Authors/Creators
- W. Li (Author/Creator)L. Huang (Author/Creator)N. Liu (Author/Creator)M.K. Pandey (Author/Creator)Y. Chen (Author/Creator)L. Cheng (Author/Creator)J. Guo (Author/Creator)B. Yu (Author/Creator)H. Luo (Author/Creator)X. Zhou (Author/Creator)D. Huai (Author/Creator)W. Chen (Author/Creator)L. Yan (Author/Creator)X. Wang (Author/Creator)Y. Lei (Author/Creator)R.K. Varshney (Author/Creator)B. Liao (Author/Creator)H. Jiang (Author/Creator)
- Publication Details
- International Journal of Molecular Sciences, Vol.22(14), Art. 7266
- Publisher
- MDPI AG
- Identifiers
- 991005543152107891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Centre for Crop and Food Innovation; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1209 Plant Sugar Metabolism
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry