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Journal article
LC-MS/MS method for the simultaneous quantification of thiafentanil and naltrexone in bovine muscle, liver and kidney
Journal of pharmaceutical and biomedical analysis, Vol.257, 116711
2025
PMID: 39904132
Abstract
Thiafentanil is a µ-opioid agonist used for the chemical immobilisation of a variety of ungulate species and is antagonised by the administration of naltrexone. The potential for these ungulates to be hunted for consumption by humans or predators raises concerns of drug residues in animal tissues. No analytical method to quantify tissue residue concentrations of thiafentanil has been previously reported. This research developed an LC-MS/MS method to quantify thiafentanil in bovine muscle, and both thiafentanil and naltrexone in bovine liver and kidney matrices. The analytical method was applied to quantify tissue residues in samples collected from goats 1, 2, 3, and 6 days post thiafentanil administration. The assay was validated over the calibration range 6.25–200ng/mg for thiafentanil in muscle, and 3.13–400ng/mg for thiafentanil and 57.8–7400ng/mg for naltrexone in liver and kidney. No residues above the lowest limit of quantification were detected in the injection site, longissimus dorsi muscle, liver or kidney samples collected from the goats. The reported analytical method and residue depletion data provide a foundation for future thiafentanil and naltrexone residue depletion studies in wildlife species.
Details
- Title
- LC-MS/MS method for the simultaneous quantification of thiafentanil and naltrexone in bovine muscle, liver and kidney
- Authors/Creators
- Judith T. Christie - Murdoch UniversityMieghan Bruce - Murdoch University, Centre for Biosecurity and One HealthSilke Pfitzer - Tshwane University of TechnologyLiesel Laubscher - Wildlife Pharmaceuticals, Rocky Drift, White River, South AfricaJacobus P. Raath - Wildlife Pharmaceuticals, Rocky Drift, White River, South AfricaMichael Laurence - Murdoch UniversityTracy Kellermann - Stellenbosch University
- Publication Details
- Journal of pharmaceutical and biomedical analysis, Vol.257, 116711
- Publisher
- Elsevier B.V.
- Number of pages
- 8
- Grant note
- Wildlife Pharmaceuticals Pty. Ltd.South African Medical Research Council (SAMRC) Centre for Tuberculosis ResearchNational Research Foundation (NRF) South African Research Chair Initiative (SARChI): 86949 Murdoch UniversitySouth African Veterinary Association's Wildlife Group Research Fund
This work was supported by Wildlife Pharmaceuticals Pty. Ltd., the South African Medical Research Council (SAMRC) Centre for Tuberculosis Research and National Research Foundation (NRF) South African Research Chair Initiative (SARChI grant 86949) , Murdoch University and the South African Veterinary Association's Wildlife Group Research Fund. The work was completed while holding the Murdoch University International Tuition Fee Scholarship and Murdoch University Strategic Partnership Scholarship.
- Identifiers
- 991005741586807891
- Copyright
- © 2025 The Authors.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.43 Anesthesiology
- 1.43.1642 Veterinary Anesthesia
- Web Of Science research areas
- Chemistry, Analytical
- Pharmacology & Pharmacy
- ESI research areas
- Pharmacology & Toxicology