Journal article
Laser‐facilitated epicutaneous immunotherapy with depigmented house dust mite extract alleviates allergic responses in a mouse model of allergic lung inflammation
Allergy, Vol.75(5), pp.1217-1228
2020
Abstract
Background
Skin-based immunotherapy of type 1 allergies has recently been re-investigated as an alternative for subcutaneous injections. In the current study, we employed a mouse model of house dust mite (HDM)-induced lung inflammation to explore the potential of laser-facilitated epicutaneous allergen-specific treatment.
Methods
Mice were sensitized against native Dermatophagoides pteronyssinus extract and repeatedly treated by application of depigmented D pteronyssinus extract via laser-generated skin micropores or by subcutaneous injection with or without alum. Following aerosol challenges, lung function was determined by whole-body plethysmography and bronchoalveolar lavage fluid was analyzed for cellular composition and cytokine levels. HDM-specific IgG subclass antibodies were determined by ELISA. Serum as well as cell-bound IgE was measured by ELISA, rat basophil leukemia cell assay, and ex vivo using a basophil activation test, respectively. Cultured lymphocytes were analyzed for cytokine secretion profiles and cellular polarization by flow cytometry.
Results
Immunization of mice by subcutaneous injection or epicutaneous laser microporation induced comparable IgG antibody levels, but the latter preferentially induced regulatory T cells and in general downregulated T cell cytokine production. This effect was found to be a result of the laser treatment itself, independent from extract application. Epicutaneous treatment of sensitized animals led to induction of blocking IgG, and improvement of lung function, superior compared to the effects of subcutaneous therapy. During the whole therapy schedule, no local or systemic side effects occurred.
Conclusion
Allergen-specific immunotherapy with depigmented HDM extract via laser-generated skin micropores offers a safe and effective treatment option for HDM-induced allergy and lung inflammation.
Details
- Title
- Laser‐facilitated epicutaneous immunotherapy with depigmented house dust mite extract alleviates allergic responses in a mouse model of allergic lung inflammation
- Authors/Creators
- E. Korotchenko (Author/Creator) - University of SalzburgR. Moya (Author/Creator) - R&D Allergy & Immunology Unit Laboratorios LETI S.L.U Madrid SpainS. Scheiblhofer (Author/Creator) - University of SalzburgI.A. Joubert (Author/Creator) - University of SalzburgJ. Horejs‐Hoeck (Author/Creator)M. Hauser (Author/Creator) - University of SalzburgD. Calzada (Author/Creator) - R&D Allergy & Immunology Unit Laboratorios LETI S.L.U Madrid SpainV. Iraola (Author/Creator) - R&D Allergy & Immunology Unit Laboratorios LETI S.L.U Madrid SpainJ. Carnés (Author/Creator) - R&D Allergy & Immunology Unit Laboratorios LETI S.L.U Madrid SpainR. Weiss (Author/Creator) - University of Salzburg
- Publication Details
- Allergy, Vol.75(5), pp.1217-1228
- Publisher
- Wiley-Blackwell
- Identifiers
- 991005545010307891
- Copyright
- © 2019 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.65 Allergy
- 1.65.264 Allergy Mechanisms
- Web Of Science research areas
- Allergy
- Immunology
- ESI research areas
- Immunology