Journal article
Liposome-mediated gene transfer in fish embryos
Transgenic Research, Vol.3(2), pp.116-119
1994
Abstract
Liposome-mediated gene transfer was used to introduce large DNA constructs into zygotes of African catfish. The technique is based on the delivery of recombinant bacteriophage lambda particles (or DNA-protamine complexes) into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Dechorionated zygotes and early fish embryos were treated with the transforming liposomes. Expression of the introduced reporter genes during the first three weeks of the development of the larvae was followed by measuring the activity of corresponding enzymes. These assays have indicated very efficient DNA uptake into the embryos.
Details
- Title
- Liposome-mediated gene transfer in fish embryos
- Authors/Creators
- J. Szelei (Author/Creator) - Hungarian Academy of SciencesL. Váradi (Author/Creator) - Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, Gödöllő, HungaryF. Müller (Author/Creator) - Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, Gödöllő, HungaryF. Erdélyi (Author/Creator) - Agricultural Biotechnology InstituteL. Orbán (Author/Creator) - Agricultural Biotechnology InstituteL. Horváth (Author/Creator) - Laboratory of Biotechnology, Department of Animal Genetics, Institute for Animal Breeding and Reproduction, University for Agricultural Sciences, Gödöllő, HungaryE. Duda (Author/Creator) - Hungarian Academy of Sciences
- Publication Details
- Transgenic Research, Vol.3(2), pp.116-119
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005544709907891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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