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Journal article
LncRNA5251 inhibits spermatogenesis via modification of cell-cell junctions
Biology direct, Vol.18(1), 31
2023
PMID: 37316926
Abstract
Background
Male factors-caused decline in total fertility has raised significant concern worldwide. LncRNAs have been identified to play various roles in biological systems, including spermatogenesis. This study aimed to explore the role of lncRNA5251 in mouse spermatogenesis.
Methods
The expression of lncRNA5251 was modulated in mouse testes in vivo or spermatogonial stem cells (C18-4 cells) in vitro by shRNA.
Results
The sperm motility in two generations mice after modulation of lncRNA5251 (muF0 and muF1) was decreased significantly after overexpression of lncRNA5251. GO enrichment analysis found that knockdown lncRNA5251 increased the expression of genes related to cell junctions, and genes important for spermatogenesis in mouse testes. Meanwhile, overexpressing lncRNA5251 decreased the gene and/or protein expression of important genes for spermatogenesis and immune pathways in mouse testes. In vitro, knockdown lncRNA5251 increased the expression of genes for cell junction, and the protein levels of some cell junction proteins such as CX37, OCLN, JAM1, VCAM1 and CADM2 in C18-4 cells. LncRNA5251 is involved in spermatogenesis by modulation of cell junctions.
Conclusion
This will provide a theoretical basis for improving male reproductive ability via lncRNA.
Details
- Title
- LncRNA5251 inhibits spermatogenesis via modification of cell-cell junctions
- Authors/Creators
- Cong Zhang - Qingdao Agricultural UniversityDongxin Lu - Qingdao Agricultural UniversityTong Niu - Qingdao Agricultural UniversityZhongyi Sun - Shenzhen UniversityYandi Wang - Qingdao Agricultural UniversityXiao Han - Qingdao Agricultural UniversityBohui Xiong - Qingdao, 266109 P. R. ChinaWei Shen - Qingdao Agricultural UniversityQingyuan Sun - Guangdong Provincial People's HospitalYong Zhao - Qingdao, 266109 P. R. China Perth, 6150 AustraliaWeidong Zhang - Qingdao Agricultural UniversityYanni Feng - Qingdao Agricultural University
- Publication Details
- Biology direct, Vol.18(1), 31
- Publisher
- BioMed Central
- Identifiers
- 991005585960707891
- Copyright
- © 2023 The Author(s).
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.960 Spermatogenesis
- Web Of Science research areas
- Biology
- ESI research areas
- Biology & Biochemistry