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Journal article
Localization of BDNF mRNA with the Huntington's disease protein in rat brain
Molecular Neurodegeneration, Vol.5(22)
2010
Abstract
Background
Studies have implicated reduced levels of brain-derived neurotrophic factor (BDNF) in the pathogenesis of Huntington's disease. Mutant huntingtin (Htt) protein was previously reported to decrease BDNF gene transcription and axonal transport of BDNF. We recently showed that wild-type Htt is associated with the Argonaute 2 microRNA-processing enzyme involved in gene silencing. In dendrites, Htt co-localizes with components of neuronal granules and mRNAs, indicating that it might play a role in post-transcriptional processing/transport of dendritic mRNAs.
Results
We conducted imaging experiments in cultured cortical neurons to demonstrate the co-localization of endogenous Htt and BDNF mRNA in fixed cells, and co-trafficking of BDNF 3'UTR mRNA with endogenous and fluorescently tagged Htt in live neurons. We used an enhanced technique that combines FISH and immunofluorescent staining to co-localize BDNF mRNA with Htt, Ago2, CPEB and dynein in thick vibratome sections of the rat cortex.
Conclusions
In cultured neurons and sections of the rat cortex, we found BDNF mRNA associated with Htt and components of neuronal RNA granules, which are centers for regulating RNA transport and local translation. Htt may play a role in post-transcriptional transport/targeting of mRNA for BDNF, thus contributing to neurotrophic support and neuron survival.
Details
- Title
- Localization of BDNF mRNA with the Huntington's disease protein in rat brain
- Authors/Creators
- B. Ma (Author/Creator) - New York UniversityB.P Culver (Author/Creator) - New York UniversityG. Baj (Author/Creator) - University of TriesteE. Tongiorgi (Author/Creator) - University of TriesteM.V Chao (Author/Creator) - New York UniversityN. Tanese (Author/Creator) - New York University
- Publication Details
- Molecular Neurodegeneration, Vol.5(22)
- Publisher
- Biomed Central
- Identifiers
- 991005546003607891
- Copyright
- © 2010 Ma et al
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.52 Neurodegenerative Diseases
- 1.52.951 Huntington's and Ataxias
- Web Of Science research areas
- Neurosciences
- ESI research areas
- Neuroscience & Behavior