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Locked nucleic acid nucleoside triphosphates and polymerases: On the way towards evolution of LNA aptamers
Journal article   Peer reviewed

Locked nucleic acid nucleoside triphosphates and polymerases: On the way towards evolution of LNA aptamers

R.N. Veedu and J. Wengel
Molecular BioSystems, Vol.5(8)
2009
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Abstract

Among numerous nucleic acid analogs reported in the past decades, locked nucleic acid (LNA) has received substantial attention and has become a significant tool within chemical biology disciplines like molecular biology research, diagnostics and therapeutic development. However, despite their obvious structurally unique properties, LNA-based aptamers for diagnostic and therapeutic applications remain largely unexplored. Future evolution of LNAoligonucleotideaptamers will depend on scientific breakthroughs relating to enzymatic polymerization using LNAnucleoside triphosphates as substrates. Herein, we highlight recent developments in this direction using various polymerases.

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