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Long-range PCR amplification as an alternative strategy for characterizing novel HLA-B alleles
Journal article   Peer reviewed

Long-range PCR amplification as an alternative strategy for characterizing novel HLA-B alleles

M.D. Curran, F. Williams, J.A.P. Earle, B.K. Rima, M.G. Dam, M. Bunce and D. Middleton
European Journal of Immunogenetics, Vol.23(4), pp.297-309
1996
DOI: https://doi.org/10.1111/j.1744-313X.1996.tb00125.x
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Abstract

We have developed a simple, rapid and reliable method for specifically amplifying and cloning full-length HLA-B genes from genomic DNA. Using this methodology we characterized three alleles of interest at the molecular level. Two of the alleles appeared in our routine class I PCR-SSOP typing system, a variant of B*5801 found in the Daudi cell line and RCE 56 and a variant of B*4101 found in a number of volunteer donors on our Bone Marrow Donor Registry. The third, a variant B35 allele found in RCE 80, was first identified as unusual by serology. Our sequencing analysis of exon 2 and exon 3 identified two of these alleles as the recently reported novel HLA-B*5802 and HLA-B*4102 alleles, while the third represents a new B35 allele officially designated B*3513.

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Source: InCites

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Collaboration types
Domestic collaboration
Citation topics
1 Clinical & Life Sciences
1.6 Immunology
1.6.607 MHC Diversity
Web Of Science research areas
Genetics & Heredity
Immunology
ESI research areas
Immunology
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