Files and links (1)
Open Access CC BY V4.0
Journal article
Long-term biological variability and the generation of a new reference interval for plasma N-terminal pro-B-type natriuretic peptide in Labrador retrievers
Journal of small animal practice, Vol.61(6), pp.368-373
2020
PMID: 32297329
Abstract
Objectives
First, to investigate the biological variability of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in healthy Labrador retrievers and compare this with current laboratory recommendations for dilated cardiomyopathy screening. Second, to calculate a breed-specific reference interval and validate it in a retrospective cohort.
Materials and Methods
Plasma NT-proBNP was measured in 51 clinically healthy Labrador retrievers at 0, 2, 4, 6 and 8 weeks. Coefficient of variation for individual dogs over time, the coefficient of variation for the group at each time point and the index of individuality were calculated. A reference interval was derived and tested on a clinical dataset available from four UK cardiology referral centres.
Results
Median NT-proBNP was 865 pmol/L (315 to 2064 pmol/L). Mean individual coefficient of variation was 19% (95% CI: 16 to 21%) and group coefficient of variation was 43% (95% CI: 41 to 46%), with index of individuality at 0.44. The breed-specific reference interval was 275 to 2100 pmol/L. In the validation group, 93% of NT-proBNP measurements from healthy dogs were within the reference interval. NT-proBNP measurements exceeded the reference interval in 82% of dogs with dilated cardiomyopathy. The upper bound of the reference interval (2100 pmol/L) had a positive predictive value of 90% and a negative predictive value of 87% for identification of dilated cardiomyopathy in this population.
Clinical Significance
Breed-specific reference intervals might improve the diagnostic accuracy of NT-proBNP measurement. Applying the currently recommended general cut-off value to Labradors is likely to result in frequent false positives and diagnosis would be improved by application of the new breed-specific reference interval calculated here.
Details
- Title
- Long-term biological variability and the generation of a new reference interval for plasma N-terminal pro-B-type natriuretic peptide in Labrador retrievers
- Authors/Creators
- S. Gomart - Univ Bristol, Langford Vets, Bristol, Avon, EnglandD. Allaway - MARS PetCare Ltd, Waltham Ctr Pet Nutr, Waltham, EnglandM. Harrison - MARS PetCare Ltd, Waltham Ctr Pet Nutr, Waltham, EnglandD. Dickson - HeartVets, Porthcawl CF11 8DG, WalesJ. Seo - Royal Vet Coll, London AL9 7TA, EnglandL. Ferasin - Lumbry Pk Vet Specialists, Alton GU34 3HL, EnglandJ. R. Payne - Univ Bristol, Langford Vets, Bristol, Avon, EnglandM. J. Hezzell - Univ Bristol, Bristol Vet Sch, Bristol, Avon, EnglandK. Borgeat - Univ Bristol, Langford Vets, Bristol, Avon, England
- Publication Details
- Journal of small animal practice, Vol.61(6), pp.368-373
- Publisher
- John Wiley & Sons Ltd on behalf of British Small Animal Veterinary Association
- Number of pages
- 6
- Identifiers
- 991005912958707891
- Copyright
- © 2020 The Authors
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: SDGs in the Output
Metrics
1 Record Views