Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep

R. Yang; C. Jacobson; G. Gardner; I. Carmichael; A.J.D. Campbell; U. Ryan

doi:10.1016/j.tvjl.2014.08.001

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Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep

Journal article

Open access

Peer reviewed

Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep

R. Yang, C. Jacobson, G. Gardner, I. Carmichael, A.J.D. Campbell and U. Ryan

The Veterinary Journal, Vol.202(2), pp.250-254

2014

DOI: https://doi.org/10.1016/j.tvjl.2014.08.001

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Abstract

Faecal excretion of Campylobacter spp. and Salmonella enterica in sheep in Australia was determined using a quantitative multiplex PCR (qPCR) targeting the Campylobacter spp. purine biosynthesis gene (PurA) and the S. enterica outer membrane protein (ompF). The mutiplex qPCR was specific and Campylobacter spp. and S. enterica were each detected with a sensitivity of 5 organisms/μL faecal DNA extract. This multiplex qPCR was used to determine the prevalence and concentration of Campylobacter spp. and S. enterica in 3412 faecal samples collected from 1189 lambs on eight farms across South Australia (n = 2 farms), New South Wales (n = 1), Victoria (n = 2) and Western Australia (n = 3) at three sampling periods (weaning, post-weaning and pre-slaughter). The overall prevalences of Campylobacter spp. and S. enterica were 13.3% and 5.0%, respectively, with the highest prevalence for Campylobacter spp. in South Australia and the highest prevalence for S. enterica in New South Wales. Campylobacter jejuni was the only Campylobacter sp. identified from a subset of 120 positive samples sequenced at the 16S locus. S. enterica serovar Typhimurium was the only serovar of S. enterica identified from a subset of 120 positive samples sequenced at the ompF locus. Across all states, Campylobacter spp. had the highest median bacterial concentration in faeces at weaning and post-weaning (medians of 3.4 × 106 and 1.1 × 105, respectively), whereas S. enterica had the highest median bacterial concentration at pre-slaughter (1.8 × 105/g faeces).

Details

Title: Longitudinal prevalence, faecal shedding and molecular characterisation of Campylobacter spp. and Salmonella enterica in sheep
Authors/Creators: R. Yang (Author/Creator) - Murdoch University
C. Jacobson (Author/Creator) - Murdoch University
G. Gardner (Author/Creator) - Murdoch University
I. Carmichael (Author/Creator) - South Australian Research and Development Institute
A.J.D. Campbell (Author/Creator) - The University of Melbourne
U. Ryan (Author/Creator) - Murdoch University
Publication Details: The Veterinary Journal, Vol.202(2), pp.250-254
Publisher: Balliere Tindall
Identifiers: 991005540996407891
Copyright: © 2014 Elsevier Ltd.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.42 Bacteriology; 1.42.376 Salmonella and Campylobacter
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science