Low level of polymorphism detected by SSR probes in bread wheat

R.K. Varshney; P.C. Sharma; P.K. Gupta; H.S. Balyan; B. Ramesh; J.K. Roy; A. Kumar; A. Sen

doi:10.1111/j.1439-0523.1998.tb01476.x

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Low level of polymorphism detected by SSR probes in bread wheat

Journal article

Peer reviewed

Low level of polymorphism detected by SSR probes in bread wheat

R.K. Varshney, P.C. Sharma, P.K. Gupta, H.S. Balyan, B. Ramesh, J.K. Roy, A. Kumar and A. Sen

Plant Breeding, Vol.117(2), pp.182-184

1998

DOI: https://doi.org/10.1111/j.1439-0523.1998.tb01476.x

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Abstract

In-gel hybridization patterns were studied in a set of nine diverse bread wheat (Triticum aestivum L. em. Thell) genotypes using 23 simple sequence repeat (SSR) probes in combination with 14 different restriction enzymes. Multilocus fingerprints due to SSR probes, shown earlier to be characteristic of a majority of plant genomes, were not obtained and only a very low level of polymorphism was detected when using as many as 142 probe-enzyme combinations. The hybridization of a prominent solitary high molecular weight fragment (> 23 kb) with a number of SSR probes suggested the presence of these SSRs (microsatellites) within the long stretches of repeated DNA sequences. This indicates that the genome of bread wheat differs from that of other plants in the organization and distribution of SSRs and that SSR probes detect very little polymorphism.

Details

Title: Low level of polymorphism detected by SSR probes in bread wheat
Authors/Creators: R.K. Varshney (Author/Creator) - Chaudhary Charan Singh University
P.C. Sharma (Author/Creator) - Chaudhary Charan Singh University
P.K. Gupta (Author/Creator) - Chaudhary Charan Singh University
H.S. Balyan (Author/Creator) - Chaudhary Charan Singh University
B. Ramesh (Author/Creator) - Chaudhary Charan Singh University
J.K. Roy (Author/Creator) - Chaudhary Charan Singh University
A. Kumar (Author/Creator) - Chaudhary Charan Singh University
A. Sen (Author/Creator) - Chaudhary Charan Singh University
Publication Details: Plant Breeding, Vol.117(2), pp.182-184
Publisher: Blackwell Publishing
Identifiers: 991005540878707891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Biotechnology & Applied Microbiology; Plant Sciences
ESI research areas: Plant & Animal Science