Journal article
LreEF1A,, a translation elongation factor from Lilium regale, Is pivotal for cucumber mosaic virus and tobacco rattle virus infections and tolerance to salt and drought
International Journal of Molecular Sciences, Vol.21(6), Article 2083
2020
Abstract
Eukaryotic translation elongation factors are implicated in protein synthesis across different living organisms, but their biological functions in the pathogenesis of cucumber mosaic virus (CMV) and tobacco rattle virus (TRV) infections are poorly understood. Here, we isolated and characterized a cDNA clone, LreEF1A4, encoding the alpha subunit of elongation factor 1, from a CMV-elicited suppression subtractive hybridization library of Lilium regale. The infection tests using CMV remarkably increased transcript abundance of LreEF1A4; however, it also led to inconsistent expression profiles of three other LreEF1A homologs (LreEF1A1–3). Protein modelling analysis revealed that the amino acid substitutions among four LreEF1As may not affect their enzymatic functions. LreEF1A4 was ectopically overexpressed in petunia (Petunia hybrida), and transgenic plants exhibited delayed leaf and flower senescence, concomitant with increased transcription of photosynthesis-related genes and reduced expression of senescence-associated genes, respectively. A compromised resistance to CMV and TRV infections was found in transgenic petunia plants overexpressing LreEF1A4, whereas its overexpression resulted in an enhanced tolerance to salt and drought stresses. Taken together, our data demonstrate that LreEF1A4 functions as a positive regulator in viral multiplication and plant adaption to high salinity and dehydration.
Details
- Title
- LreEF1A,, a translation elongation factor from Lilium regale, Is pivotal for cucumber mosaic virus and tobacco rattle virus infections and tolerance to salt and drought
- Authors/Creators
- D. Sun (Author/Creator)X. Ji (Author/Creator)Y. Jia (Author/Creator)D. Huo (Author/Creator)S. Si (Author/Creator)L. Zeng (Author/Creator)Y. Zhang (Author/Creator)L. Niu (Author/Creator)
- Publication Details
- International Journal of Molecular Sciences, Vol.21(6), Article 2083
- Publisher
- MDPI
- Identifiers
- 991005541296807891
- Copyright
- © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre; School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
36 File views/ downloads
40 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.185 RNA Translation Dynamics
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry