Journal article
Lsamp–/– mice display lower sensitivity to amphetamine and have elevated 5-HT turnover
Biochemical and Biophysical Research Communications, Vol.430(1), pp.413-418
2013
Abstract
In mice, the limbic system-associated membrane protein (Lsamp) gene has been implicated in locomotion, anxiety, fear reaction, learning, social behaviour and adaptation. Human data links the LSAMP gene to several psychiatric disorders and completed suicide. Here, we investigated changes in major monoamine systems in mice lacking the Lsamp gene. First, the locomotor and rewarding effects of amphetamine were studied in Lsamp–/– mice and Lsamp+/+ mice. Second, monoamine levels in major brain regions in response to saline and amphetamine injections were measured and, third, the expression levels of dopamine system-related genes in the brain were studied in these mice. Lsamp–/– mice displayed lower sensitivity to amphetamine in the motility box. Likewise, in the place preference test, the rewarding effect of amphetamine was absent in Lsamp–/– mice. In all brain regions studied, Lsamp–/– mice displayed lower serotonin (5-HT) baseline levels, but a greater 5-HT turnover rate, and amphetamine increased the level of 5-HT and lowered 5-HT turnover to a greater extent in Lsamp–/– mice. Finally, Lsamp–/– mice had lower level of dopamine transporter (DAT) mRNA in the mesencephalon. In conclusion, Lsamp-deficiency leads to increased endogenous 5-HT-ergic tone and enhanced 5-HT release in response to amphetamine. Elevated 5-HT function and reduced activity of DAT are the probable reasons for the blunted effects of amphetamine in these mice. Lsamp–/– mice are a promising model to study the neurobiological mechanisms of deviant social behaviour and adaptation impairment observed in many psychiatric disorders.
Details
- Title
- Lsamp–/– mice display lower sensitivity to amphetamine and have elevated 5-HT turnover
- Authors/Creators
- J. Innos (Author/Creator)E. Leidmaa (Author/Creator)M-A Philips (Author/Creator)S. Sütt (Author/Creator)A. Alttoa (Author/Creator)J. Harro (Author/Creator)S. Kõks (Author/Creator)E. Vasar (Author/Creator)
- Publication Details
- Biochemical and Biophysical Research Communications, Vol.430(1), pp.413-418
- Publisher
- Academic Press
- Identifiers
- 991005541384507891
- Copyright
- © 2012 Elsevier Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
22 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 1 Clinical & Life Sciences
- 1.108 Molecular & Cell Biology - Cancer & Development
- 1.108.1986 NCAM
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- ESI research areas
- Biology & Biochemistry