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Journal article
MALDI-TOF MS combined with AUC method for tigecycline susceptibility testing in Escherichia coli
JAC-antimicrobial resistance, Vol.6(2), dlad119
2024
PMID: 38455378
Abstract
Objectives
The wide spread of tet(X4) gene orthologues in the environment, food, poultry and humans is causing serious tigecycline resistance. Consequently, developing a fast and universal method to detect tigecycline resistance has become increasingly important.
Methods
During 2019–2022, 116 Escherichia coli isolates were obtained from nine provinces in China. All isolates were tested for their susceptibility to antimicrobial agents by the microdilution broth method and for the tet(X4) gene by PCR. Ten tet(X4)-positive E. coli isolates were used to confirm certain conditions, including the optimal incubation time, the optimal concentration of tigecycline, and the cut-off of the relative growth (RG) value.
Results
The optimal time and concentration of tigecycline for separation of susceptible and resistant isolates was 2 h and 4 mg/L, and the RG cut-off value was 0.4. We validated whether the experiment was feasible using 116 isolates of E. coli. The method yielded a susceptibility of 94.9% (95% CI: 81.4%–99.1%) and a specificity of 96.1% (95% CI: 88.3%–99.0%).
Conclusions
This research has shown that this optical antimicrobial susceptibility testing method can rapidly differentiate between susceptible and resistant phenotypes in isolates of E. coli. In the same range as the current gold-standard methods, the clinical turnaround time is reduced from 48 h to 2.5 h. The above results suggest that the method has splendid specificity and operationality.
Details
- Title
- MALDI-TOF MS combined with AUC method for tigecycline susceptibility testing in Escherichia coli
- Authors/Creators
- Zelin Yan - Second Affiliated Hospital of Zhejiang UniversityJiapin Li - Second Affiliated Hospital of Zhejiang UniversityYanyan Hu - Second Affiliated Hospital of Zhejiang UniversityYanyan Zhang - Second Affiliated Hospital of Zhejiang UniversityYuchen Wu - Second Affiliated Hospital of Zhejiang UniversityXiaoyang Ju - Second Affiliated Hospital of Zhejiang UniversityChang Cai - School of Veterinary and Life Sciences, Murdoch University, Perth, WA, AustraliaGongxiang Chen - Second Affiliated Hospital of Zhejiang UniversityChengtao Sun - Beijing Key Laboratory of Detection Technology for Animal Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing, ChinaRong Zhang - Second Affiliated Hospital of Zhejiang University
- Publication Details
- JAC-antimicrobial resistance, Vol.6(2), dlad119
- Publisher
- Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy.
- Identifiers
- 991005649569807891
- Copyright
- © The Author(s) 2024
- Murdoch Affiliation
- College of Environmental and Life Sciences
- Language
- English
- Resource Type
- Journal article
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