Journal article
Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies
European Journal of Plant Pathology, Vol.112(3), pp.211-220
2005
Abstract
Polyclonal antiserum from mice immunized with extracellular proteins from Rhizoctonia solani inhibited pectinase and cellulase activities in cell free culture supernatants of Rhizoctonia solani. Spleen mRNA from these mice was used to construct a cDNA library from which antipectinase ScFv antibodies were isolated using phage display techniques. Soluble ScFv antibodies produced by individual clones in Escherichia coli inhibited polygalacturonase in the culture supernatants of a range of fungal pathogens, including ascomycetes, basidiomycetes, and oomycetes. The soluble antibodies also inhibited maceration of potato tissue by these pathogens.
Details
- Title
- Maceration of plant tissue by fungi is inhibited by recombinant antipectinase antibodies
- Authors/Creators
- V. Manatunga (Author/Creator)H. Sanati (Author/Creator)P. Tan (Author/Creator)P.A. O’Brien (Author/Creator)
- Publication Details
- European Journal of Plant Pathology, Vol.112(3), pp.211-220
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005545255207891
- Copyright
- © Springer 2005
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1010 Plant Cell Walls
- Web Of Science research areas
- Agronomy
- Horticulture
- Plant Sciences
- ESI research areas
- Plant & Animal Science