Mapping CMV-related immune signatures in blood, aorta and perivascular mediastinal adipose tissue

Cassia Labeb; Laventa M. Obare; Quanhu Sheng; Xiuqi Zhang; Rama Gangula; Kisyua Nthenge; Samuel S. Bailin; Mona Mashayekhi; Victoria R. Stephens; Lindsey Stolze; Stephen Priest; Jordan C. Malone; Jared M. Oakes; Curtis L. Gabriel; Elizabeth J. Phillips; Spyros A. Kalams; Simon A. Mallal; Sara Gianella; John R. Koethe; Yan Ru Su; Tarek Absi; Celestine N. Wanjalla

doi:10.1098/rstb.2024.0401

Back

Mapping CMV-related immune signatures in blood, aorta and perivascular mediastinal adipose tissue

Journal article

Open access

Peer reviewed

Mapping CMV-related immune signatures in blood, aorta and perivascular mediastinal adipose tissue

Cassia Labeb, Laventa M. Obare, Quanhu Sheng, Xiuqi Zhang, Rama Gangula, Kisyua Nthenge, Samuel S. Bailin, Mona Mashayekhi, Victoria R. Stephens, Lindsey Stolze, …

Philosophical Transactions B, Vol.380(1938), 20240401

2025

DOI: https://doi.org/10.1098/rstb.2024.0401

PMID: 41194668

Files and links (1)

pdf

Published4.82 MBDownload View

CC BY V4.0, Open Access

Abstract

Adipose Tissue

Ageing

Aorta

Cytomegalovirus

Research Articles

T Cells

Temra

Cytomegalovirus (CMV) establishes lifelong latency and is linked to immunosenescence in older and immunocompromised individuals. We hypothesize that CMV drives systemic and tissue-specific immune changes that may contribute to cardiovascular disease (CVD). Thoracic aorta, blood and perivascular mediastinal adipose tissue from cardiac surgery patients (n = 11) were processed within 30–60 min of excision. CMV IgG titres were quantified through ELISA to determine CMV status: CMV(−) (n = 4) and CMV(+) (n = 7). Immune profiling was performed using flow cytometry and single-cell RNA sequencing. Analyses included MiloR and differential gene expression. Participants (mean age 69.7 ± 8.4 years) were 80% male and 70% Caucasian. CMV(−) and CMV(+) participants had mean IgG titres of 0.038 and 13.55 IU ml−1, respectively. CD8+ T-cells expressing CD57+, GPR56+ and CX3CR1+ (CGC) were increased in the blood of CMV(+) participants. In the aorta of CMV(+) participants, CD8+ T cells and CD4+ T cells had decreased HLA-C expression and suppressed interferon-α pathways. In contrast, the TNF-α signalling pathway was increased. CMV infection shapes immune responses and in this pilot, we observed suppression of interferon-α signalling and increased TNF-α-associated pathways in the aorta. Larger studies are needed to define how CMV-driven immune remodelling contributes to CVD. This article is part of the discussion meeting issue ‘The indirect effects of cytomegalovirus infection: mechanisms and consequences.’

Details

Title: Mapping CMV-related immune signatures in blood, aorta and perivascular mediastinal adipose tissue
Authors/Creators: Cassia Labeb - Vanderbilt University Medical Center
Laventa M. Obare - Vanderbilt University Medical Center
Quanhu Sheng - Vanderbilt University
Xiuqi Zhang - Vanderbilt University Medical Center
Rama Gangula - Vanderbilt University Medical Center
Kisyua Nthenge - Vanderbilt University Medical Center
Samuel S. Bailin - Vanderbilt University Medical Center
Mona Mashayekhi - Vanderbilt University Medical Center
Victoria R. Stephens - Vanderbilt University Medical Center
Lindsey Stolze - Department of Biostatistics, Vanderbilt University Medical Center, Nashville, TN, 37232, USA
Stephen Priest - Vanderbilt University Medical Center
Jordan C. Malone - Vanderbilt University Medical Center
Jared M. Oakes - Vanderbilt University Medical Center
Curtis L. Gabriel - Vanderbilt University Medical Center
Elizabeth J. Phillips
Spyros A. Kalams - Vanderbilt University Medical Center
Simon A. Mallal
Sara Gianella - University of California San Diego
John R. Koethe - Vanderbilt University Medical Center
Yan Ru Su - Vanderbilt University Medical Center
Tarek Absi - Vanderbilt University Medical Center
Celestine N. Wanjalla - Vanderbilt University Medical Center
Publication Details: Philosophical Transactions B, Vol.380(1938), 20240401
Publisher: The Royal Society
Number of pages: 13
Grant note: 2021193 / Doris Duke Charitable Foundation (http://dx.doi.org/10.13039/100000862) National Institute of Allergy and Infectious Diseases (http://dx.doi.org/10.13039/100000060) National Center for Advancing Translational Sciences (http://dx.doi.org/10.13039/100006108) K23HL156759-01A1 / National Heart, Lung and Blood Institute (NHLBI) 1021480 / Burroughs Wellcome Fund (http://dx.doi.org/10.13039/100000861)
Identifiers: 991005830350107891
Murdoch Affiliation: Institute for Immunology and Infectious Diseases
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

14 File views/ downloads

10 Record Views

1 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.161 Virology - Identification & Sequencing; 1.161.711 Cytomegalovirus Infections
Web Of Science research areas: Biology
ESI research areas: Biology & Biochemistry