Journal article
Marker-assisted introgression of resistance to fusarium wilt race 2 in Pusa 256, an elite cultivar of desi chickpea
Molecular Genetics and Genomics, Vol.292(6), pp.1237-1245
2017
Abstract
Fusarium wilt caused by F. oxysporum f. sp. ciceris causes extensive damage to chickpea (Cicer arietinum L.) in many parts of the world. In the central part of India, pathogen race 2 (Foc 2) causes severe yield losses. We initiated molecular marker-assisted backcrossing (MABC) using desi cultivar, Vijay, as a donor to introgress resistance to this race (Foc2) in Pusa 256, another elite desi cultivar of chickpea. To confirm introgression of resistance for this race, foreground selection was undertaken using two SSR markers (TA 37 and TA110), with background selection to observe the recovery of recurrent parent genome using 45 SSRs accommodated in 8 multiplexes. F1 plants were confirmed with molecular markers and backcrossed with Pusa 256, followed by cycles of foreground and background selection at each stage to generate 161 plants in BC3F2 during the period 2009–2013. Similarly, 46 BC3F1 plants were also generated in another set during the same period. On the basis of foreground selection, 46 plants were found homozygotes in BC3F2. Among them, 17 plants recorded >91% background recovery with the highest recovery percentage of 96%. In BC3F1 also, 14 hybrid plants recorded a background recovery of >85% with the highest background recovery percentage of >94%. The identified plants were selfed to obtain 1341 BC3F3 and 2198 BC3F2 seeds which were screened phenotypically for resistance to fusarium wilt (race 2) besides doing marker analysis. Finally, 17 BC3F4 and 11 BC3F3 lines were obtained which led to identification of 5 highly resistant lines of Pusa 256 with Foc 2 gene introgressed in them. Development of these lines will help in horizontal as well as vertical expansion of chickpea in central part of India.
Details
- Title
- Marker-assisted introgression of resistance to fusarium wilt race 2 in Pusa 256, an elite cultivar of desi chickpea
- Authors/Creators
- A. Pratap (Author/Creator) - Indian Institute of Pulses ResearchS.K. Chaturvedi (Author/Creator) - Indian Institute of Pulses ResearchR. Tomar (Author/Creator) - Indian Institute of Pulses ResearchN. Rajan (Author/Creator) - Indian Institute of Pulses ResearchN. Malviya (Author/Creator) - Indian Institute of Pulses ResearchM. Thudi (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsP.R. Saabale (Author/Creator) - Indian Institute of Pulses ResearchU. Prajapati (Author/Creator) - Indian Institute of Pulses ResearchR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsN.P. Singh (Author/Creator) - Indian Institute of Pulses Research
- Publication Details
- Molecular Genetics and Genomics, Vol.292(6), pp.1237-1245
- Publisher
- Springer
- Identifiers
- 991005541162707891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics