Mast cells and γδ T cells are largely dispensable for adaptive immune responses after laser-mediated epicutaneous immunization

I.A. Joubert; D. Kovacs; S. Scheiblhofer; P. Winter; E. Korotchenko; H. Strandt; R. Weiss

doi:10.1016/j.vaccine.2019.11.051

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Mast cells and γδ T cells are largely dispensable for adaptive immune responses after laser-mediated epicutaneous immunization

Journal article

Peer reviewed

Mast cells and γδ T cells are largely dispensable for adaptive immune responses after laser-mediated epicutaneous immunization

I.A. Joubert, D. Kovacs, S. Scheiblhofer, P. Winter, E. Korotchenko, H. Strandt and R. Weiss

Vaccine, Vol.38(5), pp.1015-1024

2020

DOI: https://doi.org/10.1016/j.vaccine.2019.11.051

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Abstract

Background The skin resembles an attractive target for vaccination due to its accessibility and abundance of resident immune cells. Cells like γδ T cells and mast cells (MCs) are part of the first line of defence against exogenous threats. Despite being important mediators for eliciting TH2 immune responses after epithelial stress, γδ T cell and MC functions still remain to be completely understood. Here, we aimed to characterize their roles in shaping adaptive immune responses after laser-mediated epicutaneous immunization (EPI). Methods γδ T cell knock out, MC-depleted, and wildtype control mice were immunized with mannan-conjugated grass pollen allergen Phl p 5 (P5-MN) by laser-mediated EPI. After 2–3 immunizations, cytokine expression, T helper polarization, and antigen-specific IgG1/IgE levels were analysed. Furthermore, the local cytokine/chemokine milieu after laser microporation was determined. Results The majority of inflammatory chemokines and cytokines induced by laser treatment were not affected by the presence of γδ T cells or MCs. However, RANTES was elevated in γδ T cell knock out mice and GROα, TSLP, and IL-33 were significantly decreased after MC depletion. The absence of γδ T cells or depletion of MCs had no substantial effect on adaptive immune responses after laser-mediated EPI, except for slightly reduced IgG1 and effector T cell levels in MC-depleted mice. Conclusions γδ T cells did not play a pivotal role in shaping the humoral and cellular adaptive immune response after laser-mediated EPI. MC depletion decreased the numbers of effector T cells, indicating a potential role of MCs in the activation and maturation of T cells after EPI.

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Title: Mast cells and γδ T cells are largely dispensable for adaptive immune responses after laser-mediated epicutaneous immunization
Authors/Creators: I.A. Joubert (Author/Creator) - University of Salzburg
D. Kovacs (Author/Creator) - Technical University of Munich
S. Scheiblhofer (Author/Creator) - University of Salzburg
P. Winter (Author/Creator) - University of Salzburg
E. Korotchenko (Author/Creator) - University of Salzburg
H. Strandt (Author/Creator) - University of Salzburg
R. Weiss (Author/Creator) - University of Salzburg
Publication Details: Vaccine, Vol.38(5), pp.1015-1024
Publisher: Elsevier BV
Identifiers: 991005545023007891
Copyright: © 2019 Elsevier Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.65 Allergy; 1.65.936 Mast Cell Functions
Web Of Science research areas: Immunology; Medicine, Research & Experimental
ESI research areas: Immunology