Journal article
Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study
Rapid Communications in Mass Spectrometry, Vol.21(17), pp.2913-2917
2007
Abstract
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instrumentation has been used to analyze wheat seed gliadins as an alternative to other established methods, including sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE), high-performance liquid chromatography (HPLC), etc. The MALDI-TOF approach has shown to have many advantages such as high resolution, cost effectiveness and high throughput. MALDI-TOF-based gliadin profiles have been used for fast wheat cultivar identification. However, the genetic information represented by individual gliadin peaks has not been utilized. In this study a wheat doubled haploid population with a genetic linkage map of good coverage was used to assay individual gliadin peaks from MALDI-TOF profiles as molecular markers. Eight segregating peaks in the population were scored as polymorphic across the population. The 1 to 1 segregating ratios validated the scoring of the peaks and all peaks were mapped to the expected chromosomes or linkage groups on the available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 6D.
Details
- Title
- Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin protein peaks are useful molecular markers for wheat genetic study
- Authors/Creators
- J. Chen (Author/Creator) - Agriculture and FoodP. Lan (Author/Creator) - Agriculture and FoodA. Tarr (Author/Creator) - Agriculture and FoodY.M. Yan (Author/Creator) - Capital Normal UniversityM. Francki (Author/Creator) - Agriculture and FoodR. Appels (Author/Creator) - Agriculture and FoodW. Ma (Author/Creator) - Agriculture and Food
- Publication Details
- Rapid Communications in Mass Spectrometry, Vol.21(17), pp.2913-2917
- Publisher
- John Wiley and Sons Ltd
- Identifiers
- 991005544781107891
- Copyright
- © 2007 John Wiley & Sons, Ltd.
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Spectroscopy
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- Chemistry