Measuring protein reduction potentials using N-15 HSQC NMR spectroscopy

Samantha L. Taylor; Harriet Crawley-Snowdon; Jane L. Wagstaff; Michelle L. Rowe; Mark Shepherd; Richard A. Williamson; Mark J. Howard

doi:10.1039/c3cc38952a

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Measuring protein reduction potentials using N-15 HSQC NMR spectroscopy

Journal article

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Peer reviewed

Measuring protein reduction potentials using N-15 HSQC NMR spectroscopy

Samantha L. Taylor, Harriet Crawley-Snowdon, Jane L. Wagstaff, Michelle L. Rowe, Mark Shepherd, Richard A. Williamson and Mark J. Howard

Chemical communications (Cambridge, England), Vol.49(18), pp.1847-1849

2013

DOI: https://doi.org/10.1039/c3cc38952a

PMID: 23360928

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Abstract

Chemistry

Chemistry, Multidisciplinary

Physical Sciences

Science & Technology

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple N-15 HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxido-reductase of comparable or greater reducing strength than PDI a.

Details

Title: Measuring protein reduction potentials using N-15 HSQC NMR spectroscopy
Authors/Creators: Samantha L. Taylor - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Harriet Crawley-Snowdon - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Jane L. Wagstaff - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Michelle L. Rowe - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Mark Shepherd - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Richard A. Williamson - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Mark J. Howard - Univ Kent, Sch Bioscience, Canterbury CT2 7NJ, Kent, England
Publication Details: Chemical communications (Cambridge, England), Vol.49(18), pp.1847-1849
Publisher: Royal Society Chemistry
Identifiers: 991005587768907891
Murdoch Affiliation: Centre for Computational and Systems Medicine
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.127 Molecular & Cell Biology - Pharmacology; 1.127.973 Thiol-Disulfide Systems
Web Of Science research areas: Chemistry, Multidisciplinary
ESI research areas: Chemistry