Journal article
Mesoporous silica templated biolabels with releasable fluorophores for immunoassays
Analytical Chemistry, Vol.80(14), pp.5401-5406
2008
Abstract
A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (1000−6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was 50 times higher than the conventional dye labelled antibody system.
Details
- Title
- Mesoporous silica templated biolabels with releasable fluorophores for immunoassays
- Authors/Creators
- W. Cai (Author/Creator)I.R. Gentle (Author/Creator)G.Q. Lu (Author/Creator)J-J Zhu (Author/Creator)A. Yu (Author/Creator)
- Publication Details
- Analytical Chemistry, Vol.80(14), pp.5401-5406
- Publisher
- American Chemical Society
- Identifiers
- 991005540192007891
- Copyright
- © 2008 American Chemical Society
- Murdoch Affiliation
- School of Chemical and Mathematical Science
- Language
- English
- Resource Type
- Journal article
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