Metabolic Profiling Reveals Changes in Serum Predictive of Venous Ulcer Healing

R. T. Bergner; S. Onida; R. Velineni; K. Spagou; M. S. Gohel; M. Bouschbacher; S. Bohbot; J. Shalhoub; E. Holmes; A. H. Davies

doi:10.1097/SLA.0000000000004933

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Metabolic Profiling Reveals Changes in Serum Predictive of Venous Ulcer Healing

Journal article

Open access

Peer reviewed

Metabolic Profiling Reveals Changes in Serum Predictive of Venous Ulcer Healing

R. T. Bergner, S. Onida, R. Velineni, K. Spagou, M. S. Gohel, M. Bouschbacher, S. Bohbot, J. Shalhoub, E. Holmes and A. H. Davies

Annals of surgery, Vol.277(2), pp.e467-e474

2023

DOI: https://doi.org/10.1097/SLA.0000000000004933

PMID: 35916649

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https://journals.lww.com/annalsofsurgery/Abstract/9000/Metabolic_Profiling_Reveals_Changes_in_Serum.93544.aspxView

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Abstract

mass spectrometry

metabolic profiling

metabolomics

NMR spectroscopy

Original

venous leg ulceration

VLU

wound healing

Objective: The aim of this study was to identify potential biomarkers predictive of healing or failure to heal in a population with venous leg ulceration. Summary Background Data: Venous leg ulceration presents important physical, psychological, social and financial burdens. Compression therapy is the main treatment, but it can be painful and time-consuming, with significant recurrence rates. The identification of a reliable biochemical signature with the ability to identify nonhealing ulcers has important translational applications for disease prognostication, personalized health care and the development of novel therapies. Methods: Twenty-eight patients were assessed at baseline and at 20 weeks. Untargeted metabolic profiling was performed on urine, serum, and ulcer fluid, using mass spectrometry and nuclear magnetic resonance spectroscopy. Results: A differential metabolic phenotype was identified in healing (n = 15) compared to nonhealing (n = 13) venous leg ulcer patients. Analysis of the assigned metabolites found ceramide and carnitine metabolism to be relevant pathways. In this pilot study, only serum biofluids could differentiate between healing and nonhealing patients. The ratio of carnitine to ceramide was able to differentiate between healing phenotypes with 100% sensitivity, 79% specificity, and 91% accuracy. Conclusions: This study reports a metabolic signature predictive of healing in venous leg ulceration and presents potential translational applications for disease prognostication and development of targeted therapies.

Details

Title: Metabolic Profiling Reveals Changes in Serum Predictive of Venous Ulcer Healing
Authors/Creators: R. T. Bergner
S. Onida - Charing Cross Hospital
R. Velineni
K. Spagou
M. S. Gohel
M. Bouschbacher
S. Bohbot
J. Shalhoub - Charing Cross Hospital
E. Holmes - Murdoch University, Centre for Computational and Systems Medicine
A. H. Davies
Publication Details: Annals of surgery, Vol.277(2), pp.e467-e474
Publisher: Lippincott Williams & Wilkins
Identifiers: 991005546870007891
Murdoch Affiliation: Health Futures Institute
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.197 Molecular & Cell Biology - Mitochondria; 1.197.1196 Inborn Metabolic Errors
Web Of Science research areas: Surgery
ESI research areas: Clinical Medicine