Files and links (2)
Published (Version of Record)CC BY V4.0, Open Access
Journal article
Metabolic control of nitrogen fixation in rhizobium-legume symbioses
Science Advances, Vol.7(31), eabh2433
2021
Abstract
Rhizobia induce nodule formation on legume roots and differentiate into bacteroids, which catabolize plant-derived dicarboxylates to reduce atmospheric N2 into ammonia. Despite the agricultural importance of this symbiosis, the mechanisms that govern carbon and nitrogen allocation in bacteroids and promote ammonia secretion to the plant are largely unknown. Using a metabolic model derived from genome-scale datasets, we show that carbon polymer synthesis and alanine secretion by bacteroids facilitate redox balance in microaerobic nodules. Catabolism of dicarboxylates induces not only a higher oxygen demand but also a higher NADH/NAD+ ratio than sugars. Modeling and 13C metabolic flux analysis indicate that oxygen limitation restricts the decarboxylating arm of the tricarboxylic acid cycle, which limits ammonia assimilation into glutamate. By tightly controlling oxygen supply and providing dicarboxylates as the energy and electron source donors for N2 fixation, legumes promote ammonia secretion by bacteroids. This is a defining feature of rhizobium-legume symbioses.
Details
- Title
- Metabolic control of nitrogen fixation in rhizobium-legume symbioses
- Authors/Creators
- C.C.M. Schulte (Author/Creator)K. Borah (Author/Creator)R.M. Wheatley (Author/Creator)J.J. Terpolilli (Author/Creator)G. Saalbach (Author/Creator)N. Crang (Author/Creator)D.H. de Groot (Author/Creator)R.G. Ratcliffe (Author/Creator)N.J. Kruger (Author/Creator)A. Papachristodoulou (Author/Creator)P.S. Poole (Author/Creator)
- Publication Details
- Science Advances, Vol.7(31), eabh2433
- Publisher
- American Association for the Advancement of Science
- Identifiers
- 991005541676307891
- Copyright
- © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
50 File views/ downloads
168 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology