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CC BY V4.0, Open Access
Journal article
Metabolic engineering of bread wheat improves grain iron concentration and bioavailability
Plant biotechnology journal, Vol.17(8), pp.1514-1526
2019
PMCID: PMC6662306
PMID: 30623558
Abstract
Bread wheat (Triticum aestivum L.) is cultivated on more land than any other crop and produces a fifth of the calories consumed by humans. Wheat endosperm is rich in starch yet contains low concentrations of dietary iron (Fe) and zinc (Zn). Biofortification is a micronutrient intervention aimed at increasing the density and bioavailability of essential vitamins and minerals in staple crops; Fe biofortification of wheat has proved challenging. In this study we employed constitutive expression (CE) of the rice (Oryza sativa L.) nicotianamine synthase 2 (OsNAS2) gene in bread wheat to up-regulate biosynthesis of two low molecular weight metal chelators - nicotianamine (NA) and 2'-deoxymugineic acid (DMA) - that play key roles in metal transport and nutrition. The CE-OsNAS2 plants accumulated higher concentrations of grain Fe, Zn, NA and DMA and synchrotron X-ray fluorescence microscopy (XFM) revealed enhanced localization of Fe and Zn in endosperm and crease tissues, respectively. Iron bioavailability was increased in white flour milled from field-grown CE-OsNAS2 grain and positively correlated with NA and DMA concentrations.
Details
- Title
- Metabolic engineering of bread wheat improves grain iron concentration and bioavailability
- Authors/Creators
- Jesse T Beasley (Author) - School of BioSciences, The University of Melbourne, Melbourne, VIC, AustraliaJulien P Bonneau (Author) - School of BioSciences, The University of Melbourne, Melbourne, VIC, AustraliaTona T Sanchez-Palacios (Author)Laura T Moreno-Moyano (Author) - School of BioSciences, The University of Melbourne, Melbourne, VIC, AustraliaDamien L Callahan (Author) - School of Life and Environmental Sciences, Deakin University, Burwood, VIC, AustraliaElad Tako (Author) - Robert W. Holley Center for Agriculture and Health, USDA-ARS, Ithaca, NY, USARaymond P Glahn (Author) - Robert W. Holley Center for Agriculture and Health, USDA-ARS, Ithaca, NY, USAEnzo Lombi (Author) - Future Industries Institute, University of South Australia, Mawson Lakes, SA, AustraliaAlexander A T Johnson (Author) - School of BioSciences, The University of Melbourne, Melbourne, VIC, Australia
- Publication Details
- Plant biotechnology journal, Vol.17(8), pp.1514-1526
- Publisher
- Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
- Identifiers
- 991005632670307891
- Copyright
- © 2019 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.1474 Micronutrient Interactions
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science